ArticleBiophysical journal1999
Atomic force microscopy imaging of DNA covalently immobilized on a functionalized mica substrate.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 132 citations in OpenAlex.
- Molecular Recognition in Confined Space Elucidated with DNA Nanopores and Single-Molecule Force Microscopy.Nano letters · 2023Article
- Effect of Spherical Elements of Biosensors and Bioreactors on the Physicochemical Properties of a Peroxidase Protein.Polymers · 2021Article
- An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals.Redox biology · 2020Article
- In situ Surface Charge Density Visualization of Self-assembled DNA Nanostructures after Ion Exchange.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Article
- DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.Nature communications · 2017Article
- How can macromolecular crowding inhibit biological reactions? The enhanced formation of DNA nanoparticles.Scientific reports · 2016Article
- DNA-protein interactions: methods for detection and analysis.Molecular and cellular biochemistry · 2012Review
- A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ.Molecular biology reports · 2011Article
- Scanning force microscopy studies of X-ray-induced double-strand breaks in plasmid DNA.Journal of biological physics · 2009Article
- DNA conformation on surfaces measured by fluorescence self-interference.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Padlock oligonucleotides as a tool for labeling superhelical DNA.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
A procedure for covalent binding of DNA to a functionalized mica substrate is described. The approach is based on photochemical cross-linking of DNA to immobilized psoralen derivatives. A tetrafluorphenyl (TFP) ester of trimethyl psoralen (trioxalen) was synthesized, and the procedure to immobilize it onto a functionalized aminopropyl mica surface (AP-mica) was developed. DNA molecules were cross-linked to trioxalen moieties by UV irradiation of complexes. The steps of the sample preparation procedure were analyzed with x-ray photoelectron spectroscopy (XPS). Results from XPS show that an AP-mica surface can be formed by vapor phase deposition of silane and that this surface can be derivatized with trioxalen. The derivatized surface is capable of binding of DNA molecules such that, after UV cross-linking, they withstand a thorough rinsing with SDS. Observations with atomic force microscopy showed that derivatized surfaces remain smooth, so DNA molecules are easily visualized. Linear and circular DNA molecules were photochemically immobilized on the surface. The molecules are distributed over the surface uniformly, indicating rather even modification of AP-mica with trioxalen. Generally, the shapes of supercoiled molecules electrostatically immobilized on AP-mica and those photocross-linked on trioxalen-functionalized surfaces remain quite similar. This suggests that UV cross-linking does not induce formation of a noticeable number of single-stranded breaks in DNA molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.