ArticleBiophysical journal1999
The ADP release step of the smooth muscle cross-bridge cycle is not directly associated with force generation.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Effects of cytochalasin D on relaxation process of skinned taenia cecum and carotid artery from guinea pig.The journal of physiological sciences : JPS · 2024Article
- Double-headed binding of myosin II to F-actin shows the effect of strain on head structure.Journal of structural biology · 2023Article
- Accelerating effects of blebbistatin on relaxation process of cell membrane permeabilized trachea and taenia cecum from guinea pig.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2020Article
- The load dependence and the force-velocity relation in intact myosin filaments from skeletal and smooth muscles.American journal of physiology. Cell physiology · 2020Article
- A Novel Method of Determining the Functional Effects of a Minor Genetic Modification of a Protein.Frontiers in cardiovascular medicine · 2015Article
- The effects of Ca2+ and MgADP on force development during and after muscle length changes.PloS one · 2013Article
- Myosin IC generates power over a range of loads via a new tension-sensing mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The interrelation between mechanical characteristics of contracting muscle, cross-bridge internal structure, and the mechanism of chemomechanical energy transduction.European biophysics journal : EBJ · 2012Article
- Diversity of structural behavior in vertebrate conventional myosins complexed with actin.Journal of molecular biology · 2007Article
- Smooth muscle myosin: regulation and properties.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.Biophysical journal · 2004Article
- Lever arms and necks: a common mechanistic theme across the myosin superfamily.Journal of muscle research and cell motility · 2004Review
- Myosin isoforms show unique conformations in the actin-bound state.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The kinetic properties of smooth muscle: how a little extra weight makes myosin faster.Journal of muscle research and cell motility · 2003Review
- Substrate and product dependence of force and shortening in fast and slow smooth muscle.The Journal of general physiology · 2001Article
- Photolytic release of MgADP reduces rigor force in smooth muscle.Biophysical journal · 2001Article
- The myosin cross-bridge cycle and its control by twitchin phosphorylation in catch muscle.Biophysical journal · 2001Article
- The smooth muscle cross-bridge cycle studied using sinusoidal length perturbations.Biophysical journal · 2000Article
- Kinetic studies on the effects of ADP and ionic strength on the interaction between myosin subfragment-1 and actin: implications for load-sensitivity and regulation of the crossbridge cycle.Journal of muscle research and cell motility · 1999Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
When smooth muscle myosin subfragment 1 (S1) is bound to actin filaments in vitro, the light chain domain tilts upon release of MgADP, producing a approximately 3.5-nm axial motion of the head-rod junction (Whittaker et al., 1995. Nature. 378:748-751). If this motion contributes significantly to the power stroke, rigor tension of smooth muscle should decrease substantially in response to cross-bridge binding of MgADP. To test this prediction, we monitored mechanical properties of permeabilized strips of chicken gizzard muscle in rigor and in the presence of MgADP. For comparison, we also tested psoas and soleus muscle fibers. Any residual bound ADP was minimized by incubation in Mg2+-free rigor solution containing 15 mM EDTA. The addition of 2 mM MgADP, while keeping ionic strength and free Mg2+ concentration constant, resulted in a slight increase in rigor tension in both gizzard and soleus muscles, but a decrease in psoas muscle. In-phase stiffness monitored during small (<0.1%) 500-Hz sinusoidal length oscillations decreased in all three muscle types when MgADP was added. The changes in force and stiffness with the addition of MgADP were similar at ionic strengths from 50 to 200 mM and were reversible. The results with gizzard muscle were similar after thiophosphorylation of the regulatory light chain of myosin. These results suggest that the axial motion of smooth muscle S1 bound to actin, upon dissociation of MgADP, is not associated with force generation. The difference between the present mechanical data and previous structural studies of smooth S1 may be explained if geometrical constraints of the intact contractile filament array alter the motions of the myosin heads.
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