ArticleBiophysical journal1999
Conformational changes of the in situ nuclear pore complex.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 93 citations in OpenAlex.
- Permeability-Based Measurement of Nanobody Binding to Nuclear Pore Complexes by Transient Scanning Electrochemical Microscopy.Analytical chemistry · 2026Article
- Unveiling the complexity: assessing models describing the structure and function of the nuclear pore complex.Frontiers in cell and developmental biology · 2023Review
- The Nuclear Envelope as a Regulator of Immune Cell Function.Frontiers in immunology · 2022Review
- Nanoelectrochemical Study of Molecular Transport through the Nuclear Pore Complex.Chemical record (New York, N.Y.) · 2021Review
- The Effect of Cell Morphology on the Permeability of the Nuclear Envelope to Diffusive Factors.Frontiers in physiology · 2018Article
- Modeling of the mechano-chemical behaviour of the nuclear pore complex: current research and perspectives.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Ion permeability of the nuclear pore complex and ion-induced macromolecular permeation as studied by scanning electrochemical and fluorescence microscopy.Analytical chemistry · 2014Article
- Calcium and IP3 dynamics in cardiac myocytes: experimental and computational perspectives and approaches.Frontiers in pharmacology · 2014Review
- Activation of intracellular calcium by multiple Wnt ligands and translocation of β-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/β-catenin pathways.The Journal of biological chemistry · 2013Article
- Nanoscale mechanism of molecular transport through the nuclear pore complex as studied by scanning electrochemical microscopy.Journal of the American Chemical Society · 2013Article
- Calcium signaling in synapse-to-nucleus communication.Cold Spring Harbor perspectives in biology · 2011Review
- Calcium regulation of nucleocytoplasmic transport.Protein & cell · 2011Review
- Mitogen activated protein kinase at the nuclear pore complex.Journal of cellular and molecular medicine · 2011Article
- Signaling from the secretory granule to the nucleus: Uhmk1 and PAM.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Ion channels at the nucleus: electrophysiology meets the genome.Molecular plant · 2010Review
- Apoptosis leads to a degradation of vital components of active nuclear transport and a dissociation of the nuclear lamina.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- An historical perspective on cell mechanics.Pflugers Archiv : European journal of physiology · 2008Article
- Towards reconciling structure and function in the nuclear pore complex.Histochemistry and cell biology · 2008Review
- Biology and biophysics of the nuclear pore complex and its components.International review of cell and molecular biology · 2008Review
- Route of glucocorticoid-induced macromolecules across the nuclear envelope as viewed by atomic force microscopy.Pflugers Archiv : European journal of physiology · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By bridging the double membrane separating the cell nucleus and cytoplasm, nuclear pore complexes (NPCs) are crucial pathways for the exchange of ions, proteins, and RNA between these two cellular compartments. A structure in the central lumen of the NPC, called the nuclear transport protein, central granule, or nuclear plug, appeared to gate diffusion of intermediate-sized molecules (10-40 kDa) across the nuclear membranes. Visualization of the NPC required drying and fixation of the specimen for electron and atomic force microscopy (AFM), a requirement that has raised doubts about the physiological relevance of the observation. Here we present AFM images of the outer nuclear membranes and NPCs of Xenopus laevis oocytes under more physiological conditions. Measured under a variety of Ca2+ depletion conditions, the central granule appeared to occupy and occlude the lumen of the pore in >80% of NPCs compared to <10% in controls. In a few instances images were obtained of the same NPCs as the solution was changed from control saline to store depletion conditions, and finally to store repletion conditions. We conclude that the central lumen of the nuclear pore complex undergoes a conformational change in response to depletion of nuclear cisternal Ca2+ levels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.