ArticleApplied and environmental microbiology1999
Phosphate stress in cultures and field populations of the dinoflagellate prorocentrum minimum detected by a single-cell alkaline phosphatase assay
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 147 citations in OpenAlex.
- Transcriptomic response of marine dinoflagellate Prorocentrum cordatum to phosphorus deficiency.Scientific reports · 2025Article
- Phosphorus deficiency induces sexual reproduction in the dinoflagellate Prorocentrum cordatum.Scientific reports · 2023Article
- Variable inter and intraspecies alkaline phosphatase activity within single cells of revived dinoflagellates.The ISME journal · 2021Article
- Restoration, conservation and phytoplankton hysteresis.Conservation physiology · 2021Article
- Mobilization and Cellular Distribution of Phosphate in the DiatomFrontiers in plant science · 2020Article
- Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae.Frontiers in plant science · 2020Review
- Rapid detection of Escherichia coli in beverages using genetically engineered bacteriophage T7.AMB Express · 2019Article
- Roles of Phosphorus Sources in Microbial Community Assembly for the Removal of Organic Matters and Ammonia in Activated Sludge.Frontiers in microbiology · 2019Article
- An Experimental Insight into Extracellular Phosphatases - Differential Induction of Cell-Specific Activity in Green Algae Cultured under Various Phosphorus Conditions.Frontiers in microbiology · 2018Article
- Transcriptome Analysis of Scrippsiella trochoidea CCMP 3099 Reveals Physiological Changes Related to Nitrate Depletion.Frontiers in microbiology · 2016Article
- Light availability may control extracellular phosphatase production in turbid environments.Microbial ecology · 2015Article
- De novo assembly of Aureococcus anophagefferens transcriptomes reveals diverse responses to the low nutrient and low light conditions present during blooms.Frontiers in microbiology · 2014Article
- Identification and characterization of an extracellular alkaline phosphatase in the marine diatom Phaeodactylum tricornutum.Marine biotechnology (New York, N.Y.) · 2013Article
- Characterization of the proteomic profiles of the brown tide alga Aureoumbra lagunensis under phosphate- and nitrogen-limiting conditions and of its phosphate limitation-specific protein with alkaline phosphatase activity.Applied and environmental microbiology · 2012Article
- Dissolved Organic Phosphorus Production during Simulated Phytoplankton Blooms in a Coastal Upwelling System.Frontiers in microbiology · 2012Article
- The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response.PloS one · 2012Article
- Phosphorus cycling in the red tide incubator region of monterey bay in response to upwelling.Frontiers in microbiology · 2012Article
- Nutrient-regulated transcriptional responses in the brown tide-forming alga Aureococcus anophagefferens.Environmental microbiology · 2011Article
- Proteome changes driven by phosphorus deficiency and recovery in the brown tide-forming alga Aureococcus anophagefferens.PloS one · 2011Article
- Cold stress stimulates intracellular calcification by the coccolithophore, Emiliania huxleyi (Haptophyceae) under phosphate-deficient conditions.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkaline phosphatase activity is a common marker of phosphate stress in many phytoplankton, but it has been difficult to attribute alkaline phosphatase activity to specific organisms or groups of phytoplankton in the field with traditional biochemical procedures. A new alkaline phosphatase substrate, ELF-97 (enzyme-labeled fluorescence), shows promise in this regard. When a phosphate group is cleaved from the ELF-97 reagent, the remaining molecule precipitates near the site of enzyme activity, thus fluorescently tagging cells with alkaline phosphatase activity. We characterized ELF-97 labeling in axenic cultures of a common dinoflagellate, Prorocentrum minimum, in order to understand ELF-97 labeling dynamics when phosphate nutrition varies. Enzyme activity, as detected by ELF-97 labeling, appears to be induced in late-log- or early-stationary-phase cultures if cells are grown in low-phosphate media and is lost when phosphate-stressed cells are refed with phosphate. ELF-97 appears to label an inducible intracellular alkaline phosphatase in P. minimum based on confocal microscopy studies. This may limit the use of this reagent to organisms that lack high levels of constitutive intracellular phosphatases. After laboratory cultures were characterized, ELF-97 was used to assay field populations of P. minimum in Narragansett Bay during two 1-week periods, and 12 to 100% of the P. minimum cells were labeled. The level of cell labeling was reduced by 3 days of incubation with added inorganic phosphate. Our results indicate that ELF-97 is an excellent new tool for monitoring phytoplankton phosphate stress in the environment when the data are supported by appropriate laboratory studies.
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Registered trials
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