ArticleApplied and environmental microbiology1999
Identification of a new gene family expressed during the onset of sexual reproduction in the centric diatom Thalassiosira weissflogii.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 73 citations in OpenAlex.
- Conserved genetic markers reveal widespread diatom sexual reproduction in the global ocean.Nature communications · 2025Article
- What Was Old Is New Again: The Pennate DiatomMarine drugs · 2022Review
- Microalgae production in human urine: Fundamentals, opportunities, and perspectives.Frontiers in microbiology · 2022Review
- Article
- Characterisation of Stramenopile-specific mastigoneme proteins in Phytophthora parasitica.Protoplasma · 2019Article
- Kingdom Chromista and its eight phyla: a new synthesis emphasising periplastid protein targeting, cytoskeletal and periplastid evolution, and ancient divergences.Protoplasma · 2018Article
- Ardissonea crystallina has a type of sexual reproduction that is unusual for centric diatoms.Scientific reports · 2017Article
- Diatom flagellar genes and their expression during sexual reproduction in Leptocylindrus danicus.BMC genomics · 2017Article
- Article
- A CRISPR/Cas9 system adapted for gene editing in marine algae.Scientific reports · 2016Article
- Molecular evolution of candidate male reproductive genes in the brown algal model Ectocarpus.BMC evolutionary biology · 2016Article
- Uncovering the genetic basis for early isogamete differentiation: a case study of Ectocarpus siliculosus.BMC genomics · 2013Article
- Sex-biased gene expression in the brown alga Fucus vesiculosus.BMC genomics · 2013Article
- Whole transcriptome analysis of the silicon response of the diatom Thalassiosira pseudonana.BMC genomics · 2012Article
- Genetic engineering of algae for enhanced biofuel production.Eukaryotic cell · 2010Review
- Cross-species global proteomics reveals conserved and unique processes in Phytophthora sojae and Phytophthora ramorum.Molecular & cellular proteomics : MCP · 2008Article
- Evidence for positive selection on a sexual reproduction gene in the diatom genus Thalassiosira (Bacillariophyta).Journal of molecular evolution · 2006Article
- Long serial analysis of gene expression for gene discovery and transcriptome profiling in the widespread marine coccolithophore Emiliania huxleyi.Applied and environmental microbiology · 2006Article
- Paths toward algal genomics.Plant physiology · 2005Review
- Identification of a high-affinity phosphate transporter gene in a prasinophyte alga, Tetraselmis chui, and its expression under nutrient limitation.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An intriguing feature of the diatom life cycle is that sexual reproduction and the generation of genetic diversity are coupled to the control of cell size. A PCR-based cDNA subtraction technique was used to identify genes that are expressed as small cells of the centric diatom Thalassiosira weissflogii initiate gametogenesis. Ten genes that are up-regulated during the early stages of sexual reproduction have been identified thus far. Three of the sexually induced genes, Sig1, Sig2, and Sig3, were sequenced to completion and are members of a novel gene family. The three polypeptides encoded by these genes possess different molecular masses and charges but display many features in common: they share five highly conserved domains; they each contain three or more cysteine-rich epithelial growth factor (EGF)-like repeats; and they each display homology to the EGF-like region of the vertebrate extracellular matrix glycoprotein tenascin X. Interestingly, the five conserved domains appear in the same order in each polypeptide but are separated by variable numbers of nonconserved amino acids. SIG1 and SIG2 display putative regulatory domains within the nonconserved regions. A calcium-binding, EF-hand motif is found in SIG1, and an ATP/GTP binding motif is present in SIG2. The striking similarity between the SIG polypeptides and extracellular matrix components commonly involved in cell-cell interactions suggests that the SIG polypeptides may play a role in sperm-egg recognition. The SIG polypeptides are thus important molecular targets for determining when and where sexual reproduction occurs in the field.
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