ArticleApplied and environmental microbiology1999
Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 107 citations in OpenAlex.
- Type VI secretion system degeneration accelerates intestinal epithelial cell death in Escherichia coli O157:H7.PLoS pathogens · 2026Article
- Combating Actions of Green 2D-Materials on Gram Positive and Negative Bacteria and Enveloped Viruses.Frontiers in bioengineering and biotechnology · 2020Article
- A rapid method for separating and concentration of food-borne pathogens using elution from ready-to-eat vegetables.Iranian journal of microbiology · 2018Article
- The influence of surface chemistry on the kinetics and thermodynamics of bacterial adhesion.Scientific reports · 2018Article
- Improving detection of Shiga toxin-producing Escherichia coli by molecular methods by reducing the interference of free Shiga toxin-encoding bacteriophages.Applied and environmental microbiology · 2015Article
- Implications of free Shiga toxin-converting bacteriophages occurring outside bacteria for the evolution and the detection of Shiga toxin-producing Escherichia coli.Frontiers in cellular and infection microbiology · 2014Review
- Differential gene expression and adherence of Escherichia coli O157:H7 in vitro and in ligated pig intestines.PloS one · 2011Article
- Pepper mild mottle virus as an indicator of fecal pollution.Applied and environmental microbiology · 2009Article
- A Preliminary Investigation of Fecal Indicator Bacteria, Human Pathogens, and Source Tracking Markers in Beach Water and Sand.Environment research journal · 2009Article
- Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles.BMC microbiology · 2008Article
- Molecular detection of Leptospira interrogans in human tissues and environmental samples in a lethal case of leptospirosis.International journal of legal medicine · 2008Article
- Electrochemical detection of harmful algae and other microbial contaminants in coastal waters using hand-held biosensors.Marine pollution bulletin · 2007Article
- Cladophora (Chlorophyta) spp. harbor human bacterial pathogens in nearshore water of Lake Michigan.Applied and environmental microbiology · 2006Article
- Detection of water-borne E. coli O157 using the integrating waveguide biosensor.Biosensors & bioelectronics · 2005Article
- Identification of bacterial populations in dairy wastewaters by use of 16S rRNA gene sequences and other genetic markers.Applied and environmental microbiology · 2004Article
- Evaluation of broiler litter with reference to the microbial composition as assessed by using 16S rRNA and functional gene markers.Applied and environmental microbiology · 2003Article
- A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp.BMC infectious diseases · 2002Article
- Quantitative detection of microbial genes by using DNA microarrays.Applied and environmental microbiology · 2002Article
- Growth kinetics of Escherichia coli O157:H7 on the epicarp of fresh vegetables and fruits.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chemical composition of each O-antigen subunit in gram-negative bacteria is a reflection of the unique DNA sequences within each rfb operon. By characterizing DNA sequences contained with each rfb operon, a diagnostic serotype-specific probe to Escherichia coli O serotypes that are commonly associated with bacterial infections can be generated. Recently, from an E. coli O157:H7 cosmid library, O-antigen-positive cosmids were identified with O157-specific antisera. By using the cosmid DNAs as probes, several DNA fragments which were unique to E. coli O157 serotypes were identified by Southern analysis. Several of these DNA fragments were subcloned from O157-antigen-positive cosmids and served as DNA probes in Southern analysis. One DNA fragment within plasmid pDS306 which was specific for E. coli O157 serotypes was identified by Southern analysis. The DNA sequence for this plasmid revealed homology to two rfb genes, the first of which encodes a GDP-mannose dehydratase. These rfb genes were similar to O-antigen biosynthesis genes in Vibrio cholerae and Yersinia enterocolitica serotype O:8. An oligonucleotide primer pair was designed to amplify a 420-bp DNA fragment from E. coli O157 serotypes. The PCR test was specific for E. coli O157 serotypes. PCR detected as few as 10 cells with the O157-specific rfb oligonucleotide primers. Coupled with current enrichment protocols, O157 serotyping by PCR will provide a rapid, specific, and sensitive method for identifying E. coli O157.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.