ArticleApplied and environmental microbiology1999
Evaluation of bottlenecks in the late stages of protein secretion in Bacillus subtilis.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 114 citations in OpenAlex.
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- Antigen surface display in two novel whole genome sequenced food grade strains, Lactiplantibacillus pentosus KW1 and KW2.Microbial cell factories · 2024Article
- Automation-aided construction and characterization ofFrontiers in bioengineering and biotechnology · 2024Article
- Comparison of the Immunogenic Properties ofFrontiers in microbiology · 2022Article
- Redirected Stress Responses in a Genome-Minimized 'midimSystems · 2021Article
- Construction of a High-Expression System in Bacillus through Transcriptomic Profiling and Promoter Engineering.Microorganisms · 2020Article
- Membrane Modulation of Super-Secreting "midiFrontiers in bioengineering and biotechnology · 2020Article
- Construction of aIndian journal of microbiology · 2019Article
- CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis.Nucleic acids research · 2019Article
- Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection.Microbial cell factories · 2019Article
- Inhibition of extracellular proteases improves the production of a xylanase in Parageobacillus thermoglucosidasius.BMC biotechnology · 2019Article
- Heterologous Expression of the Marine-Derived Quorum Quenching Enzyme MomL Can Expand the Antibacterial Spectrum ofMarine drugs · 2019Article
- Bottleneck in secretion of α-amylase in Bacillus subtilis.Microbial cell factories · 2017Review
- Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 inBioMed research international · 2017Article
- Display of recombinant proteins at the surface of lactic acid bacteria: strategies and applications.Microbial cell factories · 2016Review
- Homogeneity and heterogeneity in amylase production by Bacillus subtilis under different growth conditions.Microbial cell factories · 2016Article
- Versatile vector suite for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis.Applied microbiology and biotechnology · 2015Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite a high capacity for secretion of homologous proteins, the secretion of heterologous proteins by Bacillus subtilis is frequently inefficient. In the present studies, we have investigated and compared bottlenecks in the secretion of four heterologous proteins: Bacillus lichenifomis alpha-amylase (AmyL), Escherichia coli TEM beta-lactamase (Bla), human pancreatic alpha-amylase (HPA), and a lysozyme-specific single-chain antibody. The same expression and secretion signals were used for all four of these proteins. Notably, all identified bottlenecks relate to late stages in secretion, following translocation of the preproteins across the cytoplasmic membrane. These bottlenecks include processing by signal peptidase, passage through the cell wall, and degradation in the wall and growth medium. Strikingly, all translocated HPA was misfolded, its stability depending on the formation of disulfide bonds. This suggests that the disulfide bond oxidoreductases of B. subtilis cannot form the disulfide bonds in HPA correctly. As the secretion bottlenecks differed for each heterologous protein tested, it is anticipated that the efficient secretion of particular groups of heterologous proteins with the same secretion bottlenecks will require the engineering of specifically optimized host strains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.