ArticleBritish journal of pharmacology1999
Expression of P2Y receptors in cell lines derived from the human lung.
Article in British journal of pharmacology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 84 citations in OpenAlex.
- Enhancement of a nuclear factor of activated T cells (NFAT) reporter for the study of G protein-coupled receptors.Communications biology · 2026Article
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- Silica Nanoparticles Inhibit Responses to ATP in Human Airway Epithelial 16HBE Cells.International journal of molecular sciences · 2021Article
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- Dual effects of human neutrophil peptides in a mouse model of pneumonia and ventilator-induced lung injury.Respiratory research · 2018Article
- Lung injury during LPS-induced inflammation occurs independently of the receptor P2YPurinergic signalling · 2017Article
- CARMA3 Is Critical for the Initiation of Allergic Airway Inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Bicarbonate-dependent chloride transport drives fluid secretion by the human airway epithelial cell line Calu-3.The Journal of physiology · 2012Article
- Arsenic alters ATP-dependent Ca²+ signaling in human airway epithelial cell wound response.Toxicological sciences : an official journal of the Society of Toxicology · 2011Article
- ATP-mediated activation of the NADPH oxidase DUOX1 mediates airway epithelial responses to bacterial stimuli.The Journal of biological chemistry · 2009Article
- Differential effects of cyclic and constant stress on ATP release and mucociliary transport by human airway epithelia.The Journal of physiology · 2007Article
- International Union of Pharmacology LVIII: update on the P2Y G protein-coupled nucleotide receptors: from molecular mechanisms and pathophysiology to therapy.Pharmacological reviews · 2006Review
- Ion transport regulated by protease-activated receptor 2 in human airway Calu-3 epithelia.British journal of pharmacology · 2005Article
- Mechanism of efficient transfection of the nasal airway epithelium by hypotonic shock.Gene therapy · 2005Article
- Regulation of ion transport via apical purinergic receptors in intact rabbit airway epithelium.Pflugers Archiv : European journal of physiology · 2005Article
- Cell swelling-induced ATP release is tightly dependent on intracellular calcium elevations.The Journal of physiology · 2004Article
- P2Y2-receptor-mediated activation of a contralateral, lanthanide-sensitive calcium entry pathway in the human airway epithelium.British journal of pharmacology · 2004Article
- Surprises from the airway epithelium.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- Compartmentalized autocrine signaling to cystic fibrosis transmembrane conductance regulator at the apical membrane of airway epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Differential effects of UTP and ATP on ion transport in porcine tracheal epithelium.British journal of pharmacology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Northern blotting experiments have been performed with RNA extracted from several cell lines derived from the human lung in order to detect P2Y1, P2Y2, P2Y4 and P2Y6 mRNA. We have investigated the 1HAEo- and 16HBE14o- epithelial cell lines derived from the airway epithelium, the A549 cell line displaying properties of type II alveolar epithelial cells, the CALU-3 serous cells, the 6CFSMEo- submucosal cells and the HASMSC1 airway smooth muscle cells. We have also evaluated one pancreatic epithelial cell line called CFPAC-1. These experiments revealed that P2Y2 and P2Y6 mRNA are co-expressed in the IHAEo-, 16HBE14o- and A549 epithelial cell lines. The CFPAC-1 pancreatic cell line was strongly positive for the P2Y2 receptor. No signal was obtained for the P2Y1 and P2Y4 receptors. 2. We have then performed RT-PCR experiments with specific oligonucleotides of these last two P2Y receptors with the RNA used for the Northern blotting experiments. P2Y4 mRNA was detected in five cell lines: 1HAEo-, 16HBE14o-, 6CFSMEo-, HASMSC1 and CFPAC-1. P2Y1 mRNA was only detected in the CALU-3 cell line. 3. Inositol trisphosphates assays have identified a response typical of the P2Y2 receptor in the 1HAEo- and the 16HBE14o- airway epithelial cell lines which co-express P2Y2 and P2Y6 mRNA. By contrast, the 6CFSMEo- submucosal cells expressed a UTP-specific response which displayed pharmacological characteristics compatible with the human P2Y4 receptor: in particular, there was no response to UDP or ATP and the UTP effect was totally inhibited by pertussis toxin.
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