ArticleBritish journal of pharmacology1999
In vivo pathway of thermal hyperalgesia by intrathecal administration of alpha,beta-methylene ATP in mouse spinal cord: involvement of the glutamate-NMDA receptor system.
Article in British journal of pharmacology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 101 citations in OpenAlex.
- Migraine signaling pathways: purine metabolites that regulate migraine and predispose migraineurs to headache.Molecular and cellular biochemistry · 2023Review
- The Role of ATP Receptors in Pain Signaling.Neurochemical research · 2022Review
- The effect of selective serotonin reuptake inhibitor (SSRI) on pain-related behavior in a rat model of neuropathic pain.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2014Article
- Altered mitochondrial ATP synthase expression in the rat dorsal root ganglion after sciatic nerve injury and analgesic effects of intrathecal ATP.Cellular and molecular neurobiology · 2014Article
- In pursuit of P2X3 antagonists: novel therapeutics for chronic pain and afferent sensitization.Purinergic signalling · 2012Article
- Role of connexin43 and ATP in long-range bystander radiation damage and oncogenesis in vivo.Oncogene · 2011Article
- P2X and NMDA receptor involvement in temporomandibular joint-evoked reflex activity in rat jaw muscles.Brain research · 2010Article
- Mechanism of stretch-activated excitatory and inhibitory responses in the lower esophageal sphincter.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Behavioral phenotypes of mice lacking purinergic P2X4 receptors in acute and chronic pain assays.Molecular pain · 2009Article
- Emerging peripheral receptor targets for deep-tissue craniofacial pain therapies.Journal of dental research · 2009Review
- Eugenol Inhibits ATP-induced P2X Currents in Trigeminal Ganglion Neurons.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2008Article
- P2X3 receptor involvement in pain states.Molecular neurobiology · 2007Review
- Prostaglandin E2 potentiation of P2X3 receptor mediated currents in dorsal root ganglion neurons.Molecular pain · 2007Article
- Activation of TRPA1 channel facilitates excitatory synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- P2 receptors and chronic pain.Purinergic signalling · 2007Article
- Localization of P2X2 and P2X3 receptors in rat trigeminal ganglion neurons.Neuroscience · 2007Article
- Pharmacology of P2X channels.Pflugers Archiv : European journal of physiology · 2006Review
- Purinoceptors as therapeutic targets for lower urinary tract dysfunction.British journal of pharmacology · 2006Review
- P2X2 knockout mice and P2X2/P2X3 double knockout mice reveal a role for the P2X2 receptor subunit in mediating multiple sensory effects of ATP.The Journal of physiology · 2005Article
- Endogenous opioid mechanisms partially mediate P2X3/P2X2/3-related antinociception in rat models of inflammatory and chemogenic pain but not neuropathic pain.British journal of pharmacology · 2005Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The aim of the present study is to characterize the role of the P2X receptor in spinal nociceptive processing in vivo. We investigated the mechanisms of the P2X receptor agonist alpha,beta-methylene ATP (alpha,betameATP)-induced modulation of acute nociceptive signalling in mouse spinal cord. 2. Intrathecal administration of alpha,betameATP produced a significant and dose-dependent thermal hyperalgesic response. This response was completely blocked by intrathecal pretreatment with the non-selective P2 receptor antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonate (PPADS) and the selective P2X1, P2X3 and P2X2-3 receptor antagonist, 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP). Pretreatment with alpha,betameATP 15, 30 and 60 min prior to administration of a second dose of alpha,betameATP diminished the alpha,betameATP-induced thermal hyperalgesia. 3. A potent agonist for the P2X1 receptor, beta,gamma-methylene-L-ATP, did not show the hyperalgesic response, indicating that the P2X1 receptor is not involved in the spinal nociceptive pathway. 4. In fura-2 experiments using mouse dorsal root ganglion (DRG) neurons, alpha,betameATP (100 microM) increased intracellular Ca2+ ([Ca2+]i). This was not produced by a second application of alpha,betameATP. The same DRG neurons also showed a marked [Ca2+]i increase in response to capsaicin (3 microM). 5. Intrathecal pretreatment with the Ca2+-dependent exocytosis inhibitor, botulinum neurotoxin B, abolished the thermal hyperalgesia by alpha,betameATP. Furthermore, thermal hyperalgesia was significantly inhibited by the N-methyl-D-aspartate (NMDA) receptor antagonists, 2-amino-5-phosphonopentanoate (APV), dizocilpine and ifenprodil. 6. These findings suggest that alpha,betameATP-induced thermal hyperalgesia may be mediated by the spinal P2X3 receptor subtype that causes unresponsiveness by repetitive agonist applications, and that alpha,betameATP (perhaps through P2X3 receptors) may evoke spinal glutamate release which, in turn, leads to the generation of thermal hyperalgesia via activation of NMDA receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.