ArticleThe Journal of physiology1999
Activation of the Na+-K+ pump by hyposmolality through tyrosine kinase-dependent Cl- conductance in Xenopus renal epithelial A6 cells.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 56 citations in OpenAlex.
- Physiological roles of chloride ions in bodily and cellular functions.The journal of physiological sciences : JPS · 2023Review
- Effects of Genistein on Common Kidney Diseases.Nutrients · 2022Review
- Interactive Actions of Aldosterone and Insulin on Epithelial NaInternational journal of molecular sciences · 2020Article
- Change in hydration indices associated with an increase in total water intake of more than 0.5 L/day, sustained over 4 weeks, in healthy young men with initial total water intake below 2 L/day.Physiological reports · 2017Article
- Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells.Scientific reports · 2017Article
- NaAnnals of translational medicine · 2016Article
- Simulation of Cl(-) Secretion in Epithelial Tissues: New Methodology Estimating Activity of Electro-Neutral Cl(-) Transporter.Frontiers in physiology · 2015Article
- Insulin is involved in transcriptional regulation of NKCC and the CFTR Cl(-) channel through PI3K activation and ERK inactivation in renal epithelial cells.The journal of physiological sciences : JPS · 2014Article
- Distinct action of flavonoids, myricetin and quercetin, on epithelial Cl⁻ secretion: useful tools as regulators of Cl⁻ secretion.BioMed research international · 2014Article
- Hypotonic shock modulates Na(+) current via a Cl(-) and Ca(2+)/calmodulin dependent mechanism in alveolar epithelial cells.PloS one · 2013Article
- Hypotonic stress upregulates β- and γ-ENaC expression through suppression of ERK by inducing MKP-1.American journal of physiology. Renal physiology · 2012Article
- Regulation of epithelial sodium transport via epithelial Na+ channel.Journal of biomedicine & biotechnology · 2011Review
- Recovery of cell volume and electrolytes of A6 cells after re-establishing isotonicity following hypotonic stress.Pflugers Archiv : European journal of physiology · 2003Article
- Hypotonic stimulation of the Na+ active transport in frog skeletal muscle: role of the cytoskeleton.The Journal of physiology · 2003Article
- The effects of PO2 upon transepithelial ion transport in fetal rat distal lung epithelial cells.The Journal of physiology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We studied the regulatory mechanism of Na+ transport by hyposmolality in renal epithelial A6 cells. 2. Hyposmolality increased (1) Na+ absorption, which was detected as an amiloride-sensitive short-circuit current (INa), (2) Na+-K+ pump activity, (3) basolateral Cl- conductance (Gb,Cl), and (4) phosphorylation of tyrosine, suggesting an increase in activity of protein tyrosine kinase (PTK). 3. A Cl- channel blocker, 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), which abolished Gb, Cl, blocked the INa by inhibiting the Na+-K+ pump without any direct effect on amiloride-sensitive Na+ channels. Diminution of Gb,Cl by Cl- replacement with a less permeable anion, gluconate, also decreased the hyposmolality-increased Na+-K+ pump activity. 4. The PTK inhibitors tyrphostin A23 and genistein induced diminution of the hyposmolality-stimulated Gb,Cl, which was associated with attenuation of the hyposmolality-increased Na+-K+ pump activity. 5. Taken together, these observations suggest that: (1) hyposmolality activates PTK; (2) the activated PTK increases Gb,Cl; and (3) the PTK-increased Gb,Cl stimulates the Na+-K+ pump. 6. This PTK-activated Gb,Cl-mediated signalling of hyposmolality is a novel pathway for stimulation of the Na+-K+ pump.
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