ArticleOncogene1999
Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 63 citations in OpenAlex.
- One-Dimensional Search Dynamics of Tumor Suppressor p53 Regulated by a Disordered C-Terminal Domain.Biophysical journal · 2017Article
- Strong preference of BRCA1 protein to topologically constrained non-B DNA structures.BMC molecular biology · 2016Article
- Biodegradable Metals for Cardiovascular Stents: from Clinical Concerns to Recent Zn-Alloys.Advanced healthcare materials · 2016Review
- p53 Specifically Binds Triplex DNA In Vitro and in Cells.PloS one · 2016Article
- Azidophenyl as a click-transformable redox label of DNA suitable for electrochemical detection of DNA-protein interactions.Chemical science · 2015Article
- Uptake, p53 pathway activation, and cytotoxic responses for Co(II) and Ni(II) in human lung cells: implications for carcinogenicity.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Preferential binding of hot spot mutant p53 proteins to supercoiled DNA in vitro and in cells.PloS one · 2013Article
- Toxicity of depleted uranium complexes is independent of p53 activity.Journal of inorganic biochemistry · 2011Article
- Advances in carcinogenic metal toxicity and potential molecular markers.International journal of molecular sciences · 2011Review
- Zinc: a promising agent in dietary chemoprevention of cancer.The Indian journal of medical research · 2010Review
- Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA.The FEBS journal · 2009Article
- Inhibition of Ape1 nuclease activity by lead, iron, and cadmium.Environmental health perspectives · 2004Article
- Article
- Role of tumor suppressor p53 domains in selective binding to supercoiled DNA.Nucleic acids research · 2002Article
- Interference by toxic metal ions with DNA repair processes and cell cycle control: molecular mechanisms.Environmental health perspectives · 2002Review
- Update on the genotoxicity and carcinogenicity of cobalt compounds.Occupational and environmental medicine · 2001Review
- Role of heteroduplex joints in the functional interactions between human Rad51 and wild-type p53.Oncogene · 2000Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently we have shown that wild-type human p53 protein binds preferentially to supercoiled (sc) DNA in vitro in both the presence and absence of the p53 consensus sequence (p53CON). This binding produces a ladder of retarded bands on an agarose gel. Using immunoblotting with the antibody DO-1, we show that the bands obtained correspond to ethidium-stained DNA, suggesting that each band of the ladder contains a DNA-p53 complex. The intensity and the number of these hands are decreased by physiological concentrations of zinc ions. At higher zinc concentrations, binding of p53 to scDNA is completely inhibited. The binding of additional zinc ions to p53 appears much weaker than the binding of the intrinsic zinc ion in the DNA binding site of the core domain. In contrast to previously published data suggesting that 100 microM zinc ions do not influence p53 binding to p53CON in a DNA oligonucleotide, we show that 5-20 microM zinc efficiently inhibits binding of p53 to p53CON in DNA fragments. We also show that relatively low concentrations of dithiothreitol but not of 2-mercaptoethanol decrease the concentration of free zinc ions, thereby preventing their inhibitory effect on binding of p53 to DNA. Nickel and cobalt ions inhibit binding of p53 to scDNA and to its consensus sequence in linear DNA fragments less efficiently than zinc; cobalt ions are least efficient, requiring >100 microM Co2+ for full inhibition of p53 binding. Modulation of binding of p53 to DNA by physiological concentrations of zinc might represent a novel pathway that regulates p53 activity in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.