ArticleEnvironmental health perspectives1999
Collection of genomic DNA by buccal swabs for polymerase chain reaction-based biomarker assays.
Article in Environmental health perspectives, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01291745 (Collection of Biological Data With Potential Prognostic Relevance in Patients With MYELODYSPLASTIC SYNDROMES), which is not on this map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Collection of Biological Data With Potential Prognostic Relevance in Patients With MYELODYSPLASTIC SYNDROMES
Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Lack of Association between Interleukin-1Disease markers · 2018Pooled it
- A transdisciplinary model integrating genetic, physiological, and psychological correlates of voluntary exercise.Health psychology : official journal of the Division of Health Psychology, American Psychological Association · 2007Trial
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- Cohort profile: a prenatal birth cohort study of intergenerational risk and resilience after conflict and forced displacement.BMJ open · 2025Article
- HLA-DQB1 Allele Polymorphism Associated with Oral Submucous Fibrosis in Hunan, China.Journal of immunology research · 2024Article
- Safety tests and clinical research on buccal and nasal microneedle swabs for genomic analysis.Frontiers in bioengineering and biotechnology · 2023Article
- A Simple, Cost-Effective, and Extraction-Free Molecular Diagnostic Test for Sickle Cell Disease Using a Noninvasive Buccal Swab Specimen for a Limited-Resource Setting.Diagnostics (Basel, Switzerland) · 2022Article
- Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Evaluation of buccal swabs for pharmacogenetics.BMC research notes · 2018Article
- Medical-grade buccal swabs versus drugstore cotton swabs: No difference in DNA yield.MethodsX · 2018Article
- Novel Marfan Syndrome-Associated Mutation in theMolecular syndromology · 2017Article
- Peri-Implant Sulcus Fluid (PISF) Matrix Metalloproteinase (MMP) -8 Levels in Peri-Implantitis.Journal of clinical and diagnostic research : JCDR · 2016Article
- Saliva DNA quality and genotyping efficiency in a predominantly elderly population.BMC medical genomics · 2016Article
- What keeps a body moving? The brain-derived neurotrophic factor val66met polymorphism and intrinsic motivation to exercise in humans.Journal of behavioral medicine · 2014Article
- Frequency and prognostic significant of CYP3A4-A-290G polymorphism in acute myeloid leukemia.Journal of advanced research · 2014Article
- World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. Fluid biospecimen collection, processing, and storage in endometriosis research.Fertility and sterility · 2014Review
- A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms.Neural regeneration research · 2014Article
- Identification of Streptococcus parasanguinis DNA contamination in human buccal DNA samples.BMC research notes · 2013Article
- Relationship of early-onset baldness to prostate cancer in African-American men.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2013Article
- Preliminary evidence for a gene-environment interaction in predicting alcohol use disorders in adolescents.Alcoholism, clinical and experimental research · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Studies in molecular and genetic epidemiology require a high-throughput, low cost, and reliable means of genomic DNA collection. Buccal (cheek) swabs have been proposed as a means of achieving these goals, but there is little information about the practical application of this approach. From January 1995 to December 1997, we processed 995 buccal swabs for use in polymerase chain reaction (PCR)-based genotype assays in the context of ongoing molecular epidemiologic studies. Six hundred forty-seven of these swabs were processed immediately after collection and 348 were received by mail. We were able to obtain at least one genotype from 99.7% (645 of 647) of fresh-processed and 97.4% (330 of 339) of mailed biosamples. A PCR success rate of 90.3% (2,546 genotypes from 2,819 assays) was achieved. Genotypes were obtained from 96.1% (1, 865 genotypes from 1,941 assays) of fresh-processed biosamples and 77.6% (681 genotypes from 878 assays) of mailed biosamples. PCR success rates at any single locus ranged from 92.6 to 98.8% (fresh-processed) and 75.5 to 79.6% (mailed). The PCR success rate among fresh-processed biosamples was significantly higher than among mailed biosamples (Fisher's exact test p < 0.0001), and more attempts were required to obtain a successful PCR result for mailed biosamples as compared to fresh-processed biosamples. For one locus (CYP3A4), a subset of mailed biosamples was purified if two or more PCR failures occurred. Additional genotypes were obtained in 58.3% of these previously failed biosamples. Time from biosample receipt to DNA extraction had no effect on PCR success. After storage of processed biosamples for as long as 3 years, there was no appreciable decrease in the rate of PCR success. These results suggest that adequate DNA for PCR-based applications can be obtained from buccal swabs, but sampling or processing considerations may be important in obtaining optimal results.
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