ArticleProceedings of the National Academy of Sciences of the United States of America1999
Relationship between presynaptic calcium transients and postsynaptic currents at single gamma-aminobutyric acid (GABA)ergic boutons.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Presynaptic maturation of inhibitory connections onto vasoactive intestinal polypeptide-expressing GABAergic interneurons in the mouse barrel field.Pflugers Archiv : European journal of physiology · 2025Article
- Methods of measuring presynaptic function with fluorescence probes.Applied microscopy · 2021Review
- Single Synapse Indicators of Impaired Glutamate Clearance Derived from Fast iGluThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Imaging and Analysis of Presynaptic Calcium Influx in Cultured Neurons Using synGCaMP6f.Frontiers in synaptic neuroscience · 2019Article
- Chronic stress dampens excitatory synaptic gain in the paraventricular nucleus of the hypothalamus.The Journal of physiology · 2018Article
- Presynaptic mechanisms controlling calcium-triggered transmitter release at the neuromuscular junction.Current opinion in physiology · 2018Article
- A modulatory role of ASICs on GABAergic synapses in rat hippocampal cell cultures.Molecular brain · 2016Article
- ATP from synaptic terminals and astrocytes regulates NMDA receptors and synaptic plasticity through PSD-95 multi-protein complex.Scientific reports · 2016Article
- Synaptic ribbons influence the size and frequency of miniature-like evoked postsynaptic currents.Neuron · 2013Article
- Efficient maximum likelihood estimation of kinetic rate constants from macroscopic currents.PloS one · 2011Article
- Functional hallmarks of GABAergic synapse maturation and the diverse roles of neurotrophins.Frontiers in cellular neuroscience · 2011Article
- Lack of synapsin I reduces the readily releasable pool of synaptic vesicles at central inhibitory synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- The influence of multivesicular release and postsynaptic receptor saturation on transmission at granule cell to Purkinje cell synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- beta-Adrenoceptor-mediated long-term up-regulation of the release machinery at rat cerebellar GABAergic synapses.The Journal of physiology · 2005Article
- Regulation of GABA release by depolarisation-evoked Ca2+ transients at a single hippocampal terminal.Pflugers Archiv : European journal of physiology · 2004Article
- Spatial Distribution of Calcium Entry Evoked by Single Action Potentials within the Presynaptic Active Zone.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Paired-pulse depression of unitary quantal amplitude at single hippocampal synapses.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Gephyrin is critical for glycine receptor clustering but not for the formation of functional GABAergic synapses in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Intraterminal Ca2+ concentration and asynchronous transmitter release at single GABAergic boutons in rat collicular cultures.The Journal of physiology · 2003Article
- Presynaptic and postsynaptic mechanisms underlie paired pulse depression at single GABAergic boutons in rat collicular cultures.The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Postsynaptic responses to stereotyped activation of single axons are known to fluctuate, but the origin of synaptic variability in the vertebrate central nervous system is still unclear. To test the hypothesis that fluctuations of inhibitory postsynaptic currents reflect variations in presynaptic Ca2+ concentration, we examined single GABAergic axodendritic contacts in low-density cultures. Collicular neurons from rat embryos were loaded with the Ca2+ indicator Oregon Green 488 BAPTA-1. Presynaptic axon terminals were visualized by staining with the styryl dye RH414. Under the condition of action potential block, RH414-labeled boutons were activated selectively by current pulses applied through a fine-tipped glass pipette. Short (1- to 3-ms) depolarization of isolated boutons resulted in stimulus-locked changes of presynaptic Ca2+ concentration ([Ca2+]pre) and in evoked inhibitory postsynaptic currents (eIPSCs). Varying the strength of the stimulating currents produced a wide amplitude range of both presynaptic fluorescence transients (up to 220% of the resting value) and postsynaptic conductance changes (up to 2-3 nS). It was found that average eIPSCs displayed an approximately third-power dependency on [Ca2+]pre. Transmitter release retained its probabilistic character throughout the range of observed [Ca2+]pre values. In any tested single bouton, maximal eIPSCs occurred in association with the largest [Ca2+]pre transients, but failures were present at any [Ca2+]pre. The increase of maximal eIPSC amplitudes in connection with higher [Ca2+]pre supports the hypothesis that GABAergic boutons have the capacity to regulate synaptic strength by changing the number of simultaneously released vesicles.
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