ArticleProceedings of the National Academy of Sciences of the United States of America1999
Conformational variants of class II MHC/peptide complexes induced by N- and C-terminal extensions of minimal peptide epitopes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Synthetic High-density Lipoprotein Nanodiscs for Personalized Immunotherapy Against Gliomas.Clinical cancer research : an official journal of the American Association for Cancer Research · 2020Article
- Amino acid polymorphisms in the fibronectin-binding repeats of fibronectin-binding protein A affect bond strength and fibronectin conformation.The Journal of biological chemistry · 2017Article
- Immunogenic peptide discovery in cancer genomes.Current opinion in genetics & development · 2015Review
- Prediction of peptides binding to MHC class I and II alleles by temporal motif mining.BMC bioinformatics · 2013Article
- Structural Insights Into HLA-DM Mediated MHC II Peptide Exchange.Current topics in biochemical research · 2011Article
- Development of designed site-directed pseudopeptide-peptido-mimetic immunogens as novel minimal subunit-vaccine candidates for malaria.Molecules (Basel, Switzerland) · 2010Review
- A novel approach to identify antigens recognized by CD4 T cells using complement-opsonized bacteria expressing a cDNA library.Leukemia · 2005Article
- A pH-sensitive histidine residue as control element for ligand release from HLA-DR molecules.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Paradoxical intrathymic positive selection in mice with only a covalently presented agonist peptide.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Class II MHC molecules are known to exist in conformational variants. "Floppy" and "compact" forms of murine MHC molecules, for example, are discriminated by their migration behavior on SDS/PAGE and represent empty and ligand-loaded forms. Here we show that formation of distinctly faster-migrating ligand complexes (F-forms) rather than the normal compact (C-) forms of HLA-DR1 or -DR4 results from extensions of minimal peptide epitopes (such as HA306-318 or IC106-120) by approximately 10 amino acids at either the N or the C terminus. Two similar but distinct F-forms (FI and FII) were detected, depending on the site of the extension. Both F-forms were characterized by increased surface hydrophobicity and reduced SDS-stability. Native gel separations and size exclusion chromatography indicated that the F-forms had increased hydrodynamic radii compared with the C-form and an apparent size similar to that of empty MHC molecules. The regions on the ligand overhangs responsible for the effect began at a distance of approximately 5 amino acids on either side of the epitopes, comprised 4-8 amino acids (i.e., a total overhang of 9-14), and did not have a particular sequence preference. The possible functional significance of these forms is discussed.
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