ArticleProceedings of the National Academy of Sciences of the United States of America1999
Stimulation of homologous recombination in plants by expression of the bacterial resolvase ruvC.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 76 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Article
- Holliday junction resolution by At-HIGLE: an SLX1 lineage endonuclease from Arabidopsis thaliana with a novel in-built regulatory mechanism.Nucleic acids research · 2022Article
- Stimulation of homologous recombination in plants expressing heterologous recombinases.BMC plant biology · 2020Article
- Structural Aspects of DNA Repair and Recombination in Crop Improvement.Frontiers in genetics · 2020Review
- Challenges and Perspectives in Homology-Directed Gene Targeting in Monocot Plants.Rice (New York, N.Y.) · 2019Review
- Analysis of the recombination landscape of hexaploid bread wheat reveals genes controlling recombination and gene conversion frequency.Genome biology · 2019Article
- Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants.Frontiers in plant science · 2016Article
- High frequency Agrobacterium tumefaciens-mediated plant transformation induced by ammonium nitrate.Plant cell reports · 2009Article
- Targeted transgene integration in plant cells using designed zinc finger nucleases.Plant molecular biology · 2009Article
- Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ).Animal biotechnology · 2008Article
- Increased frequency of homologous recombination and T-DNA integration in Arabidopsis CAF-1 mutants.The EMBO journal · 2006Article
- Green light for gene targeting in plants.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- High-frequency gene targeting in Arabidopsis plants expressing the yeast RAD54 gene.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Nuclear-gene targeting by using single-stranded DNA avoids illegitimate DNA integration in Chlamydomonas reinhardtii.Eukaryotic cell · 2005Article
- Enhancing gene targeting efficiency in higher plants: rice is on the move.Transgenic research · 2005Review
- Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022.Plant molecular biology · 2005Article
- Gene replacement by homologous recombination in plants.Plant molecular biology · 2002Review
- RecA stimulates sister chromatid exchange and the fidelity of double-strand break repair, but not gene targeting, in plants transformed by Agrobacterium.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Gene therapy in plants.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted gene disruption exploits homologous recombination (HR) as a powerful reverse genetic tool, for example, in bacteria, yeast, and transgenic knockout mice, but it has not been applied to plants, owing to the low frequency of HR and the lack of recombinogenic mutants. To increase the frequency of HR in plants, we constructed transgenic tobacco lines carrying the Escherichia coli RuvC gene fused to a plant viral nuclear localization signal. We show that RuvC, encoding an endonuclease that binds to and resolves recombination intermediates (Holliday junctions) is properly transcribed in these lines and stimulates HR. We observed a 12-fold stimulation of somatic crossover between genomic sequences, a 11-fold stimulation of intrachromosomal recombination, and a 56-fold increase for the frequency of extrachromosomal recombination between plasmids cotransformed into young leaves via particle bombardment. This stimulating effect may be transferred to any plant species to obtain recombinogenic plants and thus constitutes an important step toward gene targeting.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.