ArticleProceedings of the National Academy of Sciences of the United States of America1999
Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 159 citations in OpenAlex.
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- Predicting human mRNA isoform levels from site-specific splicing kineticsbioRxiv : the preprint server for biology · 2026Article
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- Dynamics and evolutionary conservation of B complex protein recruitment during spliceosome activation.Nucleic acids research · 2025Article
- Dynamics and Evolutionary Conservation of B Complex Protein Recruitment During Spliceosome Activation.bioRxiv : the preprint server for biology · 2024Article
- A role for SNU66 in maintaining 5' splice site identity during spliceosome assembly.RNA (New York, N.Y.) · 2024Article
- Systems level analysis of time and stimuli specific signaling through PKA.Molecular biology of the cell · 2024Article
- MDF is a conserved splicing factor and modulates cell division and stress response inLife science alliance · 2023Article
- Isolation of mutant alleles of the U6 snRNA mmicroPublication biology · 2023Article
- Molecular interaction between small nuclear ribonucleoprotein polypeptide G and heat shock protein 70.14: a microscale thermophoresis exposition towards developing anti-cancer drugs.American journal of translational research · 2022Article
- Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery.American journal of translational research · 2021Article
- The Renpenning syndrome-associated protein PQBP1 facilitates the nuclear import of splicing factor TXNL4A through the karyopherin β2 receptor.The Journal of biological chemistry · 2020Article
- Structural dynamics of the N-terminal domain and the Switch loop of Prp8 during spliceosome assembly and activation.Nucleic acids research · 2018Review
- RocSampler: regularizing overlapping protein complexes in protein-protein interaction networks.BMC bioinformatics · 2017Article
- Structure of a pre-catalytic spliceosome.Nature · 2017Article
- Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor.BMC evolutionary biology · 2017Article
- Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae.Protein science : a publication of the Protein Society · 2016Article
- Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex.The EMBO journal · 2015Article
- Protein Composition of Infectious Spores Reveals Novel Sexual Development and Germination Factors in Cryptococcus.PLoS genetics · 2015Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The yeast U4/U6.U5 pre-mRNA splicing small nuclear ribonucleoprotein (snRNP) is a 25S small nuclear ribonucleoprotein particle similar in size, composition, and morphology to its counterpart in human cells. The yeast U4/U6.U5 snRNP complex has been purified to near homogeneity by affinity chromatography and preparative glycerol gradient sedimentation. We show that there are at least 24 proteins stably associated with this particle and performed mass spectrometry microsequencing to determine their identities. In addition to the seven canonical core Sm proteins, there are a set of U6 snRNP specific Sm proteins, eight previously described U4/U6.U5 snRNP proteins, and four novel proteins. Two of the novel proteins have likely RNA binding properties, one has been implicated in the cell cycle, and one has no identifiable sequence homologues or functional motifs. The purification of the low abundance U4/U6.U5 snRNP from yeast and the powerful sequencing methodologies using small amounts of protein make possible the rapid identification of novel and previously unidentified components of large, low-abundance macromolecular machines from any genetically manipulable organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.