ArticleInfection and immunity1999
Role of lactosyl glycan sequences in inhibiting enteropathogenic Escherichia coli attachment.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- Pathogen-induced secretory diarrhea and its prevention.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Review
- Human milk glycosaminoglycans inhibit in vitro the adhesion of Escherichia coli and Salmonella fyris to human intestinal cells.Pediatric research · 2016Article
- BfpI, BfpJ, and BfpK Minor Pilins Are Important for the Function and Biogenesis of Bundle-Forming Pili Expressed by Enteropathogenic Escherichia coli.Journal of bacteriology · 2015Article
- Caenorhabditis elegans bacterial pathogen resistant bus-4 mutants produce altered mucins.PloS one · 2014Article
- Nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharides.Trends in microbiology · 2010Review
- N-acetyllactosamine-induced retraction of bundle-forming pili regulates virulence-associated gene expression in enteropathogenic Escherichia coli.Molecular microbiology · 2010Article
- Interactions of enteropathogenic Escherichia coli with pediatric and adult intestinal biopsy specimens during early adherence.Infection and immunity · 2009Article
- From alpha to beta: identification of amino acids required for the N-acetyllactosamine-specific lectin-like activity of bundlin.Molecular microbiology · 2009Article
- The bundlin pilin protein of enteropathogenic Escherichia coli is an N-acetyllactosamine-specific lectin.Cellular microbiology · 2008Article
- Prebiotic galactooligosaccharides reduce adherence of enteropathogenic Escherichia coli to tissue culture cells.Infection and immunity · 2006Article
- N-acetyllactosamine conjugated to gold nanoparticles inhibits enteropathogenic Escherichia coli colonization of the epithelium in human intestinal biopsy specimens.Infection and immunity · 2006Article
- Lactoferrin and free secretory component of human milk inhibit the adhesion of enteropathogenic Escherichia coli to HeLa cells.BMC microbiology · 2001Article
- Expression of intimin gamma from enterohemorrhagic Escherichia coli in Citrobacter rodentium.Infection and immunity · 2000Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we found that asialo-lactosamine sequences served as receptors for enteropathogenic Escherichia coli (EPEC) binding to Chinese hamster ovary (CHO) cells. In the present report, we have extended these earlier results by examining the ability of lactosamine- or fucosylated lactosamine-bovine serum albumin (BSA) glycoconjugates to inhibit EPEC, strain E2348/69, binding to HEp-2 cells. We found that, consistent with our previous findings with CHO cells, N-acetyllactosamine-BSA was the most effective inhibitor of EPEC localized adherence to HEp-2 cells, with Lewis X-BSA being the next best inhibitor. Further investigation revealed that coincubating EPEC E2348/69 with these BSA glycoconjugates alone caused a decrease in the expression of the bundle-forming pilus structural subunit (BfpA) and intimin by the bacteria. BfpA and intimin expression were reduced to the greatest extent by N-acetyllactosamine-BSA and Lewis X-BSA, respectively. These results suggest that the glycoconjugate inhibition of EPEC binding to HEp-2 cells might be achieved, wholly or in part, by an active mechanism that is distinct from simple competitive antagonism of receptor-adhesin interactions.
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