ArticleInfection and immunity1999
Pili binding to asialo-GM1 on epithelial cells can mediate cytotoxicity or bacterial internalization by Pseudomonas aeruginosa.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Comparison of virulence factors genes between exoU and exoS Pseudomonas aeruginosa in microbial keratitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Molecular Mechanisms Underlying the Pathogenicity ofMedicina (Kaunas, Lithuania) · 2026Review
- Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis.Nature communications · 2025Article
- Evidence for intracellularJournal of bacteriology · 2024Review
- A Polyclonal Antibody against aVaccines · 2024Article
- Synergistic Effect of Plant Compounds in Combination with Conventional Antimicrobials against Biofilm ofPharmaceuticals (Basel, Switzerland) · 2023Review
- Pseudomonas aeruginosa type IV pili actively induce mucus contraction to form biofilms in tissue-engineered human airways.PLoS biology · 2023Article
- Pf4 Phage Variant Infection Reduces Virulence-Associated Traits inMicrobiology spectrum · 2022Article
- Chimeric Ligands of Pili and Lectin A Inhibit Tolerance, Persistence, and Virulence Factors ofACS infectious diseases · 2022Article
- Bacterial Biofilms and Their Implications in Pathogenesis and Food Safety.Foods (Basel, Switzerland) · 2021Review
- Identification of cell-surface glycans that mediate motility-dependent binding and internalization of Pseudomonas aeruginosa by phagocytes.Molecular immunology · 2021Article
- Searching for the Secret of Stickiness: How Biofilms Adhere to Surfaces.Frontiers in microbiology · 2021Review
- Article
- Super-Resolution Microscopy Reveals Local Accumulation of Plasma Membrane Gangliosides atFrontiers in cell and developmental biology · 2019Article
- Hetero-Multivalency of Pseudomonas aeruginosa Lectin LecA Binding to Model Membranes.Scientific reports · 2018Article
- Pathogenomics of Virulence Traits ofFrontiers in microbiology · 2018Article
- Cryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution.Structure (London, England : 1993) · 2017Article
- Blue light treatment of Pseudomonas aeruginosa: Strong bactericidal activity, synergism with antibiotics and inactivation of virulence factors.Virulence · 2017Article
- Acidosis increases the susceptibility of respiratory epithelial cells toAmerican journal of physiology. Lung cellular and molecular physiology · 2017Article
- Highly differentiated human airway epithelial cells: a model to study host cell-parasite interactions in pertussis.Infectious diseases (London, England) · 2016Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
The interaction of Pseudomonas aeruginosa type IV pili and the glycosphingolipid asialo-GM1 (aGM1) can mediate bacterial adherence to epithelial cells, but the steps subsequent to this adherence have not been elucidated. To investigate the result of the interaction of pili and aGM1, we used polarized epithelial monolayers of Madin-Darby canine kidney (MDCK) cells in culture, which contained little detectable aGM1 on their apical surface but were able to incorporate exogenous aGM1. Compared to an untreated monolayer, P. aeruginosa PA103 displayed an eightfold increase in association with and fivefold more cytotoxicity toward MDCK cells pretreated with aGM1. Cytotoxicity of either carrier-treated or aGM1-treated monolayers required the type III secreted protein ExoU. Asialo-GM1 pretreatment of MDCK monolayers likewise augmented bacterial internalization of an isogenic invasive strain approximately fourfold. These increases were not seen in monolayers treated with GM1, the sialyated form of the glycolipid, and were inhibited by treatment with an antibody to aGM1. Also, the aGM1-mediated adhesion, cytotoxicity, and internalization required intact type IV pili since nonpiliated PA103 mutants were unaffected by aGM1 pretreatment of MDCK cells. These results demonstrate that epithelial cell injury and bacterial internalization can proceed from the same adhesin-receptor interaction, and they indicate that P. aeruginosa exoproducts solely determine the steps subsequent to adhesion.
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