ArticleInfection and immunity1999
Unanticipated heterogeneity in growth rate and virulence among Candida albicans AAF1 null mutants.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- The δ subunit of FNature communications · 2021Article
- Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis.Scientific reports · 2019Article
- Candida albicans-Induced Epithelial Damage Mediates Translocation through Intestinal Barriers.mBio · 2018Article
- The Anti-Adhesive Effect of Curcumin onFrontiers in microbiology · 2017Article
- An assessment of growth media enrichment on lipid metabolome and the concurrent phenotypic properties of Candida albicans.PloS one · 2014Article
- Candida albicans SRR1, a putative two-component response regulator gene, is required for stress adaptation, morphogenesis, and virulence.Eukaryotic cell · 2011Article
- Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity.Nature genetics · 2010Article
- A multifunctional mannosyltransferase family in Candida albicans determines cell wall mannan structure and host-fungus interactions.The Journal of biological chemistry · 2010Article
- Gene overexpression/suppression analysis of candidate virulence factors of Candida albicans.Eukaryotic cell · 2008Article
- Candida albicans Ecm33p is important for normal cell wall architecture and interactions with host cells.Eukaryotic cell · 2006Article
- The Aspergillus fumigatus StuA protein governs the up-regulation of a discrete transcriptional program during the acquisition of developmental competence.Molecular biology of the cell · 2005Article
- Ectopic expression of URA3 can influence the virulence phenotypes and proteome of Candida albicans but can be overcome by targeted reintegration of URA3 at the RPS10 locus.Eukaryotic cell · 2004Article
- Rfg1, a protein related to the Saccharomyces cerevisiae hypoxic regulator Rox1, controls filamentous growth and virulence in Candida albicans.Molecular and cellular biology · 2001Article
- Candida albicans RIM101 pH response pathway is required for host-pathogen interactions.Infection and immunity · 2000Article
- Defective hyphal development and avirulence caused by a deletion of the SSK1 response regulator gene in Candida albicans.Infection and immunity · 2000Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The disruption of a specific gene in Candida albicans is commonly used to determine the function of the gene product. We disrupted AAF1, a gene of C. albicans that causes Saccharomyces cerevisiae to flocculate and adhere to endothelial cells. We then characterized multiple heterozygous and homozygous mutants. These null mutants adhered to endothelial cells to the same extent as did the parent organism. However, mutants with presumably the same genotype revealed significant heterogeneity in their growth rates in vitro. This heterogeneity was not the result of the transformation procedure per se, nor was it caused by differences in the expression or function of URA3, a marker used in the process of gene disruption. The growth rate among the different heterozygous and homozygous null mutants was positively correlated with in vivo virulence in mice. It is possible that the variable phenotypes of C. albicans were due to mutations outside of the AAF1 coding region that were introduced during the gene disruption process. These results indicate that careful phenotypic characterization of mutants of C. albicans generated through targeted gene disruption should be performed to exclude the introduction of unexpected mutations that may influence pathogenicity in mice.
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