ArticleBritish journal of cancer1999
Differentiation of human colon cancer cells changes the expression of beta-tubulin isotypes and MAPs.
Article in British journal of cancer, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 57 citations in OpenAlex.
- Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells.Life science alliance · 2022Article
- Circulating microRNA sequencing revealed miRNome patterns in hematology and oncology patients aiding the prognosis of invasive aspergillosis.Scientific reports · 2022Article
- Bax/Tubulin/Epithelial-Mesenchymal Pathways Determine the Efficacy of Silybin Analog HM015k in Colorectal Cancer Cell Growth and Metastasis.Frontiers in pharmacology · 2018Article
- Expression of ERCC1, RRM1, TUBB3 in correlation with apoptosis repressor ARC, DNA mismatch repair proteins and p53 in liver metastasis of colorectal cancer.International journal of molecular medicine · 2017Article
- Texture Analysis of Abnormal Cell Images for Predicting the Continuum of Colorectal Cancer.Analytical cellular pathology (Amsterdam) · 2017Article
- Differential in vitro sensitivity to patupilone versus paclitaxel in uterine and ovarian carcinosarcoma cell lines is linked to tubulin-beta-III expression.Gynecologic oncology · 2012Article
- Differential expression of glu-tubulin in relation to mammary gland disease.Virchows Archiv : an international journal of pathology · 2010Article
- TP53 status and taxane-platinum versus platinum-based therapy in ovarian cancer patients: a non-randomized retrospective study.BMC cancer · 2008Article
- Paclitaxel resistance in untransformed human mammary epithelial cells is associated with an aneuploidy-prone phenotype.British journal of cancer · 2007Article
- The roles of beta-tubulin mutations and isotype expression in acquired drug resistance.Cancer informatics · 2007Article
- Mechanisms of Taxol resistance related to microtubules.Oncogene · 2003Review
- Taxotere resistance in SUIT Taxotere resistance in pancreatic carcinoma cell line SUIT 2 and its sublines.World journal of gastroenterology · 2001Article
- Resistance to Taxol in lung cancer cells associated with increased microtubule dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human colon adenocarcinoma HT29-D4 cell line is an interesting model for studies on epithelial cell differentiation. Undifferentiated cells are malignant proliferating cells, whereas differentiated cells act like epithelial polarized cells. In the present study, we first characterized the action of taxoids on the microtubular network of HT29-D4 cells according to the state of differentiation. Microtubular bundles were found in undifferentiated cells but not in differentiated cells, even with 500-fold higher taxoid concentrations for 96 h. This finding led us to study changes in microtubules according to the polarity status of the cell. E-MAP-115 was expressed only in differentiated cells; expression of beta-tubulin isotypes was altered in them relative to undifferentiated cells. Classes I, II, III, IVa and IVb isotypes were expressed in both phenotypes; however, differentiated epithelial cells displayed a specific increase in class III beta-tubulin. Thus, the increase in expression of this beta-tubulin isotype in differentiated cells is not restricted to neuronal cells. Moreover, these expression changes may reflect a higher stability of microtubular network in differentiated cells, which may explain the lower activity of anti-microtubule agents, independently of the mitotic process. These results indicate that the composition of microtubules should be considered as one of the criteria involved in the response of tumour cells to chemotherapy with anti-microtubule agents.
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