ArticleMolecular and cellular biology1999
Activating phosphorylation of the Kin28p subunit of yeast TFIIH by Cak1p.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 49 citations in OpenAlex.
- Structural basis of Cdk7 activation by dual T-loop phosphorylation.Nature communications · 2024Article
- Structural basis of Cdk7 activation by dual T-loop phosphorylation.bioRxiv : the preprint server for biology · 2024Article
- Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes.The Plant journal : for cell and molecular biology · 2019Article
- Cdk7: a kinase at the core of transcription and in the crosshairs of cancer drug discovery.Transcription · 2019Review
- Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.Nucleic acids research · 2014Article
- The CDK Network: Linking Cycles of Cell Division and Gene Expression.Genes & cancer · 2012Article
- Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation.Molecular and cellular biology · 2010Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- The Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II.Molecular and cellular biology · 2006Article
- Kinase Cak1 functionally interacts with the PAF1 complex and phosphatase Ssu72 via kinases Ctk1 and Bur1.Molecular genetics and genomics : MGG · 2006Article
- Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo.Molecular and cellular biology · 2006Article
- Phosphorylation by Cak1 regulates the C-terminal domain kinase Ctk1 in Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription.The EMBO journal · 2005Article
- Histone H2B ubiquitylation is associated with elongating RNA polymerase II.Molecular and cellular biology · 2005Article
- The plant-specific kinase CDKF;1 is involved in activating phosphorylation of cyclin-dependent kinase-activating kinases in Arabidopsis.The Plant cell · 2004Article
- Article
- The Cdk-activating kinase Cak1p promotes meiotic S phase through Ime2p.Molecular and cellular biology · 2003Article
- Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast.The EMBO journal · 2003Article
- Feminizing chicks: a model for avian sex determination based on titration of Hint enzyme activity and the predicted structure of an Asw-Hint heterodimer.Genome biology · 2003Article
- Activation of the Bur1-Bur2 cyclin-dependent kinase complex by Cak1.Molecular and cellular biology · 2002Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Cyclin-dependent kinase (CDK)-activating kinases (CAKs) carry out essential activating phosphorylations of CDKs such as Cdc2 and Cdk2. The catalytic subunit of mammalian CAK, MO15/Cdk7, also functions as a subunit of the general transcription factor TFIIH. However, these functions are split in budding yeast, where Kin28p functions as the kinase subunit of TFIIH and Cak1p functions as a CAK. We show that Kin28p, which is itself a CDK, also contains a site of activating phosphorylation on Thr-162. The kinase activity of a T162A mutant of Kin28p is reduced by approximately 75 to 80% compared to that of wild-type Kin28p. Moreover, cells containing kin28(T162A) and a conditional allele of TFB3 (the ortholog of the mammalian MAT1 protein, an assembly factor for MO15 and cyclin H) are severely compromised and display a significant further reduction in Kin28p activity. This finding provides in vivo support for the previous biochemical observation that MO15-cyclin H complexes can be activated either by activating phosphorylation of MO15 or by binding to MAT1. Finally, we show that Kin28p is no longer phosphorylated on Thr-162 following inactivation of Cak1p in vivo, that Cak1p can phosphorylate Kin28p on Thr-162 in vitro, and that this phosphorylation stimulates the CTD kinase activity of Kin28p. Thus, Kin28p joins Cdc28p, the major cell cycle Cdk in budding yeast, as a physiological Cak1p substrate. These findings indicate that although MO15 and Cak1p constitute different forms of CAK, both control the cell cycle and the phosphorylation of the C-terminal domain of the large subunit of RNA polymerase II by TFIIH.
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