ArticleMolecular and cellular biology1999
SDF-2 induction of terminal differentiation in Dictyostelium discoideum is mediated by the membrane-spanning sensor kinase DhkA.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Diverse Roles of the Multiple Phosphodiesterases in the Regulation of Cyclic Nucleotide Signaling inCells · 2025Review
- The Acrasis kona genome and developmental transcriptomes reveal deep origins of eukaryotic multicellular pathways.Nature communications · 2024Article
- Novel RNAseq-Informed Cell-type Markers and Their Regulation Alter Paradigms ofFrontiers in cell and developmental biology · 2022Article
- From environmental sensing to developmental control: cognitive evolution in dictyostelid social amoebas.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- The Journey from Two-Step to Multi-Step Phosphorelay Signaling Systems.Current genomics · 2021Review
- Cytokinins in Dictyostelia - A Unique Model for Studying the Functions of Signaling Agents From Species to Kingdoms.Frontiers in cell and developmental biology · 2020Review
- Diversity and Evolution of Sensor Histidine Kinases in Eukaryotes.Genome biology and evolution · 2019Article
- Extracellular signaling in Dictyostelium.The International journal of developmental biology · 2019Review
- Evolution of multicellularity in Dictyostelia.The International journal of developmental biology · 2019Review
- Cell-type specific RNA-Seq reveals novel roles and regulatory programs for terminally differentiated Dictyostelium cells.BMC genomics · 2018Article
- The polymorphic proteins TgrB1 and TgrC1 function as a ligand-receptor pair inJournal of cell science · 2017Article
- Secreted Cyclic Di-GMP Induces Stalk Cell Differentiation in the Eukaryote Dictyostelium discoideum.Journal of bacteriology · 2016Review
- The Evolution of Aggregative Multicellularity and Cell-Cell Communication in the Dictyostelia.Journal of molecular biology · 2015Review
- Genetic control of morphogenesis in Dictyostelium.Developmental biology · 2015Review
- Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation.BMC genomics · 2015Article
- Cell signaling during development of Dictyostelium.Developmental biology · 2014Review
- The cyclic AMP phosphodiesterase RegA critically regulates encystation in social and pathogenic amoebas.Cellular signalling · 2014Article
- Structural basis for cytokinin receptor signaling: an evolutionary approach.Plant cell reports · 2013Review
- The polyketide MPBD initiates the SDF-1 signaling cascade that coordinates terminal differentiation in Dictyostelium.Eukaryotic cell · 2011Article
- Evolution of developmental cyclic adenosine monophosphate signaling in the Dictyostelia from an amoebozoan stress response.Development, growth & differentiation · 2011Review
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5 authors.
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Abstract
SDF-2 is a peptide released by prestalk cells during culmination that stimulates prespore cells to encapsulate. Genetic evidence indicates that the response is dependent on the dhkA gene. This gene encodes a member of the histidine kinase family of genes that functions in two-component signal transduction pathways. The sequence of the N-terminal half of DhkA predicts two hydrophobic domains separated by a 310-amino-acid loop that could bind a ligand. By inserting MYC6 epitopes into DhkA, we were able to show that the loop is extracellular while the catalytic domain is cytoplasmic. Cells expressing the MYC epitope in the extracellular domain of DhkA were found to respond only if induced with 100-fold-higher levels of SDF-2 than required to induce dhkA+ cells; however, they could be induced to sporulate by addition of antibodies specific to the MYC epitope. To examine the enzymatic activity of DhkA, we purified the catalytic domain following expression in bacteria and observed incorporation of labelled phosphate from ATP consistent with histidine autophosphorylation. Site-directed mutagenesis of histidine1395 to glutamine in the catalytic domain blocked autophosphorylation. Furthermore, genetic analyses showed that histidine1395 and the relay aspartate2075 of DhkA are both critical to its function but that another histidine kinase, DhkB, can partially compensate for the lack of DhkA activity. Sporulation is drastically reduced in double mutants lacking both DhkA and DhkB. Suppressor studies indicate that the cyclic AMP (cAMP) phosphodiesterase RegA and the cAMP-dependent protein kinase PKA act downstream of DhkA.
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