ArticleMolecular and cellular biology1999
Dominant negative murine serum response factor: alternative splicing within the activation domain inhibits transactivation of serum response factor binding targets.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 92 citations in OpenAlex.
- ScIsoX: a multidimensional framework for measuring isoform-level transcriptomic complexity in single cells.Genome biology · 2025Article
- The transcription factor SRF regulates MERVL retrotransposons and gene expression during zygotic genome activation.Genes & development · 2025Article
- SRF: a seriously responsible factor in cardiac development and disease.Journal of biomedical science · 2022Review
- O-GlcNAcylation inhibits hepatic stellate cell activation.Journal of gastroenterology and hepatology · 2021Article
- Serum response factor deletion 5 regulates phospholamban phosphorylation and calcium uptake.Journal of molecular and cellular cardiology · 2021Article
- The Aging Vasculature: Glucose Tolerance, Hypoglycemia and the Role of the Serum Response Factor.Journal of cardiovascular development and disease · 2021Review
- SRF and SRFΔ5 Splicing Isoform Recruit Corepressor LSD1/KDM1A Modifying Structural Neuroplasticity and Environmental Stress Response.Molecular neurobiology · 2020Article
- SRF regulates craniofacial development through selective recruitment of MRTF cofactors by PDGF signaling.Developmental cell · 2014Article
- Neurokinin B induces c-fos transcription via protein kinase C and activation of serum response factor and Elk-1 in immortalized GnRH neurons.Endocrinology · 2014Article
- RNA binding proteins in the regulation of heart development.The international journal of biochemistry & cell biology · 2013Review
- Article
- Mutual antagonism between IP(3)RII and miRNA-133a regulates calcium signals and cardiac hypertrophy.The Journal of cell biology · 2012Article
- SOX9 and myocardin counteract each other in regulating vascular smooth muscle cell differentiation.Biochemical and biophysical research communications · 2012Article
- Cleavage of serum response factor mediated by enteroviral protease 2A contributes to impaired cardiac function.Cell research · 2012Article
- Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover.Developmental biology · 2011Article
- Myocardin-dependent activation of the CArG box-rich smooth muscle gamma-actin gene: preferential utilization of a single CArG element through functional association with the NKX3.1 homeodomain protein.The Journal of biological chemistry · 2009Article
- Embryological origin of airway smooth muscle.Proceedings of the American Thoracic Society · 2008Review
- Variety is the splice of life.Journal of molecular and cellular cardiology · 2008Article
- Evidence for alternative splicing of MADS-box transcripts in developing cotton fibre cells.Molecular genetics and genomics : MGG · 2008Article
- Serum response factor and myocardin mediate arterial hypercontractility and cerebral blood flow dysregulation in Alzheimer's phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Primary transcripts encoding the MADS box superfamily of proteins, such as MEF2 in animals and ZEMa in plants, are alternatively spliced, producing several isoformic species. We show here that murine serum response factor (SRF) primary RNA transcripts are alternatively spliced at the fifth exon, deleting approximately one-third of the C-terminal activation domain. Among the different muscle types examined, visceral smooth muscles have a very low ratio of SRFDelta5 to SRF. Increased levels of SRFDelta5 correlates well with reduced smooth muscle contractile gene activity within the elastic aortic arch, suggesting important biological roles for differential expression of SRFDelta5 variant relative to wild-type SRF. SRFDelta5 forms DNA binding-competent homodimers and heterodimers. SRFDelta5 acts as a naturally occurring dominant negative regulatory mutant that blocks SRF-dependent skeletal alpha-actin, cardiac alpha-actin, smooth alpha-actin, SM22alpha, and SRF promoter-luciferase reporter activities. Expression of SRFDelta5 interferes with differentiation of myogenic C2C12 cells and the appearance of skeletal alpha-actin and myogenin mRNAs. SRFDelta5 repressed the serum-induced activity of the c-fos serum response element. SRFDelta5 fused to the yeast Gal4 DNA binding domain displayed low transcriptional activity, which was complemented by overexpression of the coactivator ATF6. These results indicate that the absence of exon 5 might be bypassed through recruitment of transcription factors that interact with extra-exon 5 regions in the transcriptional activating domain. The novel alternatively spliced isoform of SRF, SRFDelta5, may play an important regulatory role in modulating SRF-dependent gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.