ArticleThe EMBO journal1999
Partial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 71 citations in OpenAlex.
- SAP130 and CSN1 interact and regulate male gametogenesis in Arabidopsis thaliana.Journal of plant research · 2021Article
- Article
- The Spliceosomal Protein SF3B5 is a Novel Component of Drosophila SAGA that Functions in Gene Expression Independent of Splicing.Journal of molecular biology · 2016Article
- Structure-function analysis and genetic interactions of the SmG, SmE, and SmF subunits of the yeast Sm protein ring.RNA (New York, N.Y.) · 2016Article
- Structure-function analysis and genetic interactions of the Yhc1, SmD3, SmB, and Snp1 subunits of yeast U1 snRNP and genetic interactions of SmD3 with U2 snRNP subunit Lea1.RNA (New York, N.Y.) · 2015Article
- Alternative Splicing Regulation of Cancer-Related Pathways in Caenorhabditis elegans: An In Vivo Model System with a Powerful Reverse Genetics Toolbox.International journal of cell biology · 2013Review
- Composition of yeast snRNPs and snoRNPs in the absence of trimethylguanosine caps reveals nuclear cap binding protein as a gained U1 component implicated in the cold-sensitivity of tgs1Δ cells.Nucleic acids research · 2011Article
- Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing.RNA (New York, N.Y.) · 2010Article
- Enhancer of decapping proteins 1 and 2 are important for translation during heat stress in Saccharomyces cerevisiae.Molecular microbiology · 2009Article
- Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium.Protein expression and purification · 2007Article
- Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assembly.RNA (New York, N.Y.) · 2006Article
- U2 snRNA-protein contacts in purified human 17S U2 snRNPs and in spliceosomal A and B complexes.Molecular and cellular biology · 2006Article
- Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing.The EMBO journal · 2004Article
- The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing.Genome biology · 2004Article
- Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination.The EMBO journal · 2002Article
- Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2001Article
- Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo.Molecular and cellular biology · 2001Article
- Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation.Molecular and cellular biology · 2001Article
- UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation.The EMBO journal · 2001Article
- SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome.The EMBO journal · 2001Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have partially purified the U2 snRNP of Saccharomyces cerevisiae. Identification of some proteins consistently found in the purified fractions by nanoelectrospray mass spectrometry indicated the presence of a novel splicing factor named Rse1p. The RSE1 gene is essential and codes for a 148.2 kDa protein. We demonstrated that Rse1p associates specifically with U2 snRNA at low salt concentrations. In addition, we showed that Rse1p is a component of the pre-spliceosome. Depletion of Rse1p and analysis of a conditional mutant indicated that Rse1p was required for efficient splicing in vivo. In vitro Rse1p is required for the formation of pre-spliceosomes. Database searches revealed that Rse1p is conserved in humans and that it belongs to a large protein family that includes polyadenylation factors and DNA repair proteins. The characteristics of Rse1p suggest that its human homologue could be a subunit of the SF3 splicing factor.
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