ArticleThe EMBO journal1999
Drainin required for membrane fusion of the contractile vacuole in Dictyostelium is the prototype of a protein family also represented in man.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Structure and dynamics of the contractile vacuole complex in Tetrahymena thermophila.Journal of cell science · 2023Article
- A conserved pressure-driven mechanism for regulating cytosolic osmolarity.Current biology : CB · 2023Article
- A conserved pressure-driven mechanism for regulating cytosolic osmolarity.bioRxiv : the preprint server for biology · 2023Article
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- The C Isoform of Dictyostelium Tetraspanins Localizes to the Contractile Vacuole and Contributes to Resistance against Osmotic Stress.PloS one · 2016Article
- Evidence for Extracellular ATP as a Stress Signal in a Single-Celled Organism.Eukaryotic cell · 2015Article
- Calcium-dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel.Nature cell biology · 2014Article
- Functional properties of five Dictyostelium discoideum P2X receptors.The Journal of biological chemistry · 2013Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- A mechanism of intracellular P2X receptor activation.The Journal of biological chemistry · 2012Article
- Rab8a regulates the exocyst-mediated kiss-and-run discharge of the Dictyostelium contractile vacuole.Molecular biology of the cell · 2012Article
- Article
- Roles of an unconventional protein kinase and myosin II in amoeba osmotic shock responses.Traffic (Copenhagen, Denmark) · 2009Article
- Regulation of contractile vacuole formation and activity in Dictyostelium.The EMBO journal · 2008Article
- Copine A is required for cytokinesis, contractile vacuole function, and development in Dictyostelium.Eukaryotic cell · 2007Article
- The contractile vacuole in Ca2+-regulation in Dictyostelium: its essential function for cAMP-induced Ca2+-influx.BMC developmental biology · 2006Article
- Identification and characterization of a novel alpha-kinase with a von Willebrand factor A-like motif localized to the contractile vacuole and Golgi complex in Dictyostelium discoideum.Molecular biology of the cell · 2005Article
- Defects in cytokinesis, actin reorganization and the contractile vacuole in cells deficient in RhoGDI.The EMBO journal · 2002Article
- Genetic analysis of endocytosis in Caenorhabditis elegans: coelomocyte uptake defective mutants.Genetics · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The contractile vacuole expels water by forming a channel with the plasma membrane and thus enables cells to survive in a hypo-osmotic environment. Here we characterize drainin, a Dictyostelium protein involved in this process, as the first member of a protein family represented in fission yeast, Caenorhabditis elegans and man. Gene replacement in Dictyostelium shows that drainin acts at a checkpoint of channel formation between the contractile vacuole and the plasma membrane. A green fluorescent protein fusion of drainin localizes specifically to the contractile vacuole and rescues its periodic discharge in drainin-null cells. Drainin is a peripheral membrane protein, requiring a short hydrophobic stretch in its C-terminal region for localization and function. We suggest that drainin acts in a signaling cascade that couples a volume-sensing device in the vacuolar membrane to the membrane fusion machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.