ArticleThe EMBO journal1999
Early requirement for alpha-SNAP and NSF in the secretory cascade in chromaffin cells.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 98 citations in OpenAlex.
- ATPase N-ethylmaleimide-sensitive factor mediated calcium dependent fusion pore closure and endocytosis.Communications biology · 2026Article
- Physiology of intracellular calcium buffering.Physiological reviews · 2023Review
- α-Synuclein is required for sperm exocytosis at a post-fusion stage.Frontiers in cell and developmental biology · 2023Article
- The secreted neurotrophin Spätzle 3 promotes glial morphogenesis and supports neuronal survival and function.Genes & development · 2017Article
- A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.Frontiers in molecular neuroscience · 2017Article
- Huntingtin-associated protein 1 regulates exocytosis, vesicle docking, readily releasable pool size and fusion pore stability in mouse chromaffin cells.The Journal of physiology · 2014Article
- Article
- Neuropeptide Y and somatostatin inhibit insulin secretion through different mechanisms.American journal of physiology. Endocrinology and metabolism · 2013Article
- Article
- The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection.Plant molecular biology · 2012Article
- Modulation of endothelial SK3 channel activity by Ca²+dependent caveolar trafficking.American journal of physiology. Cell physiology · 2012Article
- Phospholipase C-related but catalytically inactive protein (PRIP) modulates synaptosomal-associated protein 25 (SNAP-25) phosphorylation and exocytosis.The Journal of biological chemistry · 2012Article
- P2X7 receptors trigger ATP exocytosis and modify secretory vesicle dynamics in neuroblastoma cells.The Journal of biological chemistry · 2011Article
- Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction.PloS one · 2009Article
- The SNAP-25 linker as an adaptation toward fast exocytosis.Molecular biology of the cell · 2008Article
- DOC2B acts as a calcium switch and enhances vesicle fusion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Ca-dependent nonsecretory vesicle fusion in a secretory cell.The Journal of general physiology · 2008Article
- A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion.Molecular biology of the cell · 2008Article
- Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Somatodendritic release of glutamate regulates synaptic inhibition in cerebellar Purkinje cells via autocrine mGluR1 activation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NSF and alpha-SNAP have been shown to be required for SNARE complex disassembly and exocytosis. However, the exact requirement for NSF and alpha-SNAP in vesicular traffic through the secretory pathway remains controversial. We performed a study on the kinetics of exocytosis from bovine chromaffin cells using high time resolution capacitance measurement and electrochemical amperometry, combined with flash photolysis of caged Ca2+ as a fast stimulus. alpha-SNAP, a C-terminal mutant of alpha-SNAP, and NEM were assayed for their effects on secretion kinetics. Two kinetically distinct components of catecholamine release can be observed upon fast step-like elevation of [Ca2+]i. One is the exocytotic burst, thought to represent the readily releasable pool of vesicles. Following the exocytotic burst, secretion proceeds slowly at maintained high [Ca2+]i, which may represent vesicle maturation/recruitment, i.e. some priming steps after docking. alpha-SNAP increased the amplitude of both the exocytotic burst and the slow component but did not change their kinetics, which we examined with millisecond time resolution. In addition, NEM only partially inhibited the slow component without altering the exocytotic burst, fusion kinetics and the rate of endocytosis. These results suggest a role for alpha-SNAP/NSF in priming granules for release at an early step, but not modifying the fusion of readily releasable granules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.