ArticleThe EMBO journal1999
Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 141 citations in OpenAlex.
- Reverse-Phase Protein Microarrays for OverexpressedAnalytical chemistry · 2024Article
- Transcriptome and metabolome profiling to elucidate the mechanism underlying the poor growth ofFrontiers in veterinary science · 2023Article
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- Surviving Serum: the Escherichia coliInfection and immunity · 2021Article
- The molecular basis of regulation of bacterial capsule assembly by Wzc.Nature communications · 2021Article
- Tyrosine phosphorylation-dependent localization of TmaR that controls activity of a major bacterial sugar regulator by polar sequestration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Tyrosine Phosphorylation as a Widespread Regulatory Mechanism in Prokaryotes.Journal of bacteriology · 2019Review
- Phenotypical profile and global transcriptomic profile of Hypervirulent Klebsiella pneumoniae due to carbapenemase-encoding plasmid acquisition.BMC genomics · 2019Article
- The GTPase BipA expressed at low temperature inThe Journal of biological chemistry · 2018Article
- Phosphorylation of Staphylococcus aureus Protein-Tyrosine Kinase Affects the Function of Glucokinase and Biofilm Formation.Iranian biomedical journal · 2017Article
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- Mass Spectrometry Offers Insight into the Role of Ser/Thr/Tyr Phosphorylation in the Mycobacteria.Frontiers in microbiology · 2016Review
- Article
- Cloning, purification, crystallization and 1.57 Å resolution X-ray data analysis of AmsI, the tyrosine phosphatase controlling amylovoran biosynthesis in the plant pathogen Erwinia amylovora.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Mycobacterium tuberculosis supports protein tyrosine phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Microbial protein-tyrosine kinases.The Journal of biological chemistry · 2014Review
- The genome sequence of the fungal pathogen Fusarium virguliforme that causes sudden death syndrome in soybean.PloS one · 2014Article
- PssP2 is a polysaccharide co-polymerase involved in exopolysaccharide chain-length determination in Rhizobium leguminosarum.PloS one · 2014Article
- The UDP-glucose dehydrogenase of Escherichia coli K-12 displays substrate inhibition by NAD that is relieved by nucleotide triphosphates.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotes, tyrosine protein phosphorylation has been studied extensively, while in bacteria, it is considered rare and is poorly defined. We demonstrate that Escherichia coli possesses a gene, etk, encoding an inner membrane protein that catalyses tyrosine autophosphorylation and phosphorylation of a synthetic co-polymer poly(Glu:Tyr). This protein tyrosine kinase (PTK) was termed Ep85 or Etk. All the E.coli strains examined possessed etk; however, only a subset of pathogenic strains expressed it. Etk is homologous to several bacterial proteins including the Ptk protein of Acinetobacter johnsonii, which is the only other known prokaryotic PTK. Other Etk homologues are AmsA of the plant pathogen Erwinia amylovora and Orf6 of the human pathogen Klebsiella pneumoniae. These proteins are involved in the production of exopolysaccharide (EPS) required for virulence. We demonstrated that like Etk, AmsA and probably also Orf6 are PTKs. Taken together, these findings suggest that tyrosine protein phosphorylation in prokaryotes is more common than was appreciated previously, and that Etk and its homologues define a distinct protein family of prokaryotic membrane-associated PTKs involved in EPS production and virulence. These prokaryotic PTKs may serve as a new target for the development of new antibiotics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.