ArticleJournal of bacteriology1999
Insertional inactivation of Treponema denticola tap1 results in a nonmotile mutant with elongated flagellar hooks.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
- Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids.Molecular oral microbiology · 2026Article
- A bipartite bacterial virulence factor targets the complement system and neutrophil activation.The EMBO journal · 2025Article
- Identification and Characterization of the Alternative σJournal of bacteriology · 2022Article
- Transcriptional and functional characterizations of multiple flagellin genes in spirochetes.Molecular microbiology · 2022Article
- Promoter activity of ORF-less gene cassettes isolated from the oral metagenome.Scientific reports · 2019Article
- A pleiotropic role of FlaG in regulating the cell morphogenesis and flagellar homeostasis at the cell poles of Treponema denticola.Cellular microbiology · 2019Article
- The Role ofFrontiers in cellular and infection microbiology · 2019Article
- A novel glycan modifies the flagellar filament proteins of the oral bacterium Treponema denticola.Molecular microbiology · 2017Article
- Spirochaete flagella hook proteins self-catalyse a lysinoalanine covalent crosslink for motility.Nature microbiology · 2016Article
- pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.Applied and environmental microbiology · 2016Article
- Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.Applied and environmental microbiology · 2015Article
- Evidence that TP_0144 of Treponema pallidum is a thiamine-binding protein.Journal of bacteriology · 2015Article
- Dioxygen and nitric oxide scavenging by Treponema denticola flavodiiron protein: a mechanistic paradigm for catalysis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2015Article
- Initial characterization of the FlgE hook high molecular weight complex of Borrelia burgdorferi.PloS one · 2014Article
- Inactivation of cyclic Di-GMP binding protein TDE0214 affects the motility, biofilm formation, and virulence of Treponema denticola.Journal of bacteriology · 2013Article
- Treponema denticola superoxide reductase: in vivo role, in vitro reactivities, and a novel [Fe(Cys)(4)] site.Biochemistry · 2012Article
- Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.Applied and environmental microbiology · 2012Article
- Treponema denticola interactions with host proteins.Journal of oral microbiology · 2012Article
- Abrogation of neuraminidase reduces biofilm formation, capsule biosynthesis, and virulence of Porphyromonas gingivalis.Infection and immunity · 2012Article
- The riboswitch regulates a thiamine pyrophosphate ABC transporter of the oral spirochete Treponema denticola.Journal of bacteriology · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The treponemal fla operon is comprised of numerous motility-related genes; however, the initial gene of this operon, tap1, has no known function. A recently developed system to generate specific mutants in Treponema denticola was utilized to determine if Tap1 was essential for motility. T. denticola tap1 and flanking DNA were identified, cloned, and sequenced, and a suicide plasmid that contained tap1 interrupted with an erythromycin resistance cassette (ermF and ermAM) was constructed. Because of potential polar effects from this cassette, a second plasmid that contained tap1 interrupted with a modified erythromycin resistance cassette that lacked the putative ermF transcription terminator was constructed. Electroporation-mediated allelic exchange incorporated the interrupted tap1 genes into the T. denticola chromosome, creating Tap1-deficient mutants. Reverse transcriptase PCR revealed that the erythromycin resistance cassette within tap1 did not terminate fla operon transcription in either mutant. Moreover, the phenotypes of the two mutants were indistinguishable. These mutants lacked motion in liquid culture, were unable to spread on agar plates, and lacked flagellar filaments as determined by electron microscopy. Immunoblots revealed a marked reduction in detectable FlaB flagellar filament protein compared to that of wild type; however, flaB RNA was easily detectable, and transcription levels did not appear to be altered. The basis for the lack of filament protein expression is unknown. Immunoblotting also showed that the flagellar hook protein (FlgE) was synthesized in the Tap1-deficient mutant; however, electron microscopy revealed that the mutant possessed unusual elongated hooks of variable lengths. We propose that treponemal Tap1 is analogous to FliK, which is involved in monitoring the flagellar hook length of Salmonella typhimurium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.