ArticleThe Journal of cell biology1999
Intron-independent association of splicing factors with active genes.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 74 citations in OpenAlex.
- Temporal gating dictates stress-induced transcript export from the nucleus.Genes & development · 2026Article
- Temporal gating dictates stress-induced transcript export from the nucleus.bioRxiv : the preprint server for biology · 2026Article
- Nuclear speckles - a driving force in gene expression.Journal of cell science · 2022Review
- Coordination of RNA Processing Regulation by Signal Transduction Pathways.Biomolecules · 2021Review
- Long Noncoding RNAs-Crucial Players Organizing the Landscape of the Neuronal Nucleus.International journal of molecular sciences · 2021Review
- Stress as a Chromatin Landscape Architect.Frontiers in cell and developmental biology · 2021Review
- Coordination of transcription, processing, and export of highly expressed RNAs by distinct biomolecular condensates.Emerging topics in life sciences · 2020Article
- Nuclear hubs built on RNAs and clustered organization of the genome.Current opinion in cell biology · 2020Review
- RNA processing errors triggered by cadmium and integrator complex disruption are signals for environmental stress.BMC biology · 2019Article
- CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci.Nucleic acids research · 2018Article
- Heat Shock Protein Genes Undergo Dynamic Alteration in Their Three-Dimensional Structure and Genome Organization in Response to Thermal Stress.Molecular and cellular biology · 2017Article
- Gene regulation: the HSP70 gene jumps when shocked.Current biology : CB · 2014Article
- HSP70 transgene directed motion to nuclear speckles facilitates heat shock activation.Current biology : CB · 2014Article
- Article
- B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress.Cell death & disease · 2013Article
- Compound A, a selective glucocorticoid receptor modulator, enhances heat shock protein Hsp70 gene promoter activation.PloS one · 2013Article
- Functional consequences of developmentally regulated alternative splicing.Nature reviews. Genetics · 2011Review
- Biotinylated quercetin as an intrinsic photoaffinity proteomics probe for the identification of quercetin target proteins.Bioorganic & medicinal chemistry · 2011Article
- Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Association of adipogenic genes with SC-35 domains during porcine adipogenesis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The cell nucleus is organized as discrete domains, often associated with specific events involved in chromosome organization, replication, and gene expression. We have examined the spatial and functional relationship between the sites of heat shock gene transcription and the speckles enriched in splicing factors in primary human fibroblasts by combining immunofluorescence and fluorescence in situ hybridization (FISH). The hsp90alpha and hsp70 genes are inducibly regulated by exposure to stress from a low basal level to a high rate of transcription; additionally the hsp90alpha gene contains 10 introns whereas the hsp70 gene is intronless. At 37 degrees C, only 30% of hsp90alpha transcription sites are associated with speckles whereas little association is detected with the hsp70 gene, whose constitutive expression is undetectable relative to the hsp90alpha gene. Upon exposure of cells to heat shock, the heavy metal cadmium, or the amino acid analogue azetidine, transcription at the hsp90alpha and hsp70 gene loci is strongly induced, and both hsp transcription sites become associated with speckles in >90% of the cells. These results reveal a clear disconnection between the presence of intervening sequences at specific gene loci and the association with splicing factor-rich regions and suggest that subnuclear structures containing splicing factors are associated with sites of transcription.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.