ArticleJournal of clinical microbiology1999
Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 97 citations in OpenAlex.
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- A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics.Journal of industrial microbiology & biotechnology · 2020Article
- Microbiological contamination of nebulizers used by cystic fibrosis patients: an underestimated problem.Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia · 2019Observational
- Community-acquired urinary tract infections caused byJMM case reports · 2017Article
- Fast and specific detection of Pseudomonas Aeruginosa from other pseudomonas species by PCR.Annals of burns and fire disasters · 2016Article
- PCR-based assay for the rapid and precise distinction of Pseudomonas aeruginosa from other Pseudomonas species recovered from burns patients.Journal of preventive medicine and hygiene · 2016Article
- Severe Community-Acquired Pneumonia with Bacteremia Caused by Herbaspirillum aquaticum or Herbaspirillum huttiense in an Immune-Competent Adult.Journal of clinical microbiology · 2015Article
- Burkholderia gladioli infection isolated from the blood cultures of newborns in the neonatal intensive care unit.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Ralstonia spp.: emerging global opportunistic pathogens.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Review
- Optical and dielectric sensors based on antimicrobial peptides for microorganism diagnosis.Frontiers in microbiology · 2014Review
- Burkholderia gladioli sepsis in newborns.European journal of pediatrics · 2012Article
- Differentiating the growing nosocomial infectious threats Ralstonia pickettii and Ralstonia insidiosa.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- A case of native valve endocarditis caused by Burkholderia cepacia without predisposing factors.BMC infectious diseases · 2011Article
- Identification of potential diagnostic markers among Burkholderia cenocepacia and B. multivorans supernatants.Journal of clinical microbiology · 2010Article
- The changing microbial epidemiology in cystic fibrosis.Clinical microbiology reviews · 2010Review
- Naturally occurring Class A ss-lactamases from the Burkholderia cepacia complex.Antimicrobial agents and chemotherapy · 2009Article
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients.Journal of clinical microbiology · 2008Article
- Direct comparison of the BD phoenix system with the MicroScan WalkAway system for identification and antimicrobial susceptibility testing of Enterobacteriaceae and nonfermentative gram-negative organisms.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cystic fibrosis (CF) predisposes patients to bacterial colonization and infection of the lower airways. Several species belonging to the genus Burkholderia are potential CF-related pathogens, but microbiological identification may be complicated. This situation is not in the least due to the poorly defined taxonomic status of these bacteria, and further validation of the available diagnostic assays is required. A total of 114 geographically diverse bacterial isolates, previously identified in reference laboratories as Burkholderia cepacia (n = 51), B. gladioli (n = 14), Ralstonia pickettii (n = 6), B. multivorans (n = 2), Stenotrophomonas maltophilia (n = 3), and Pseudomonas aeruginosa (n = 11), were collected from environmental, clinical, and reference sources. In addition, 27 clinical isolates putatively identified as Burkholderia spp. were recovered from the sputum of Dutch CF patients. All isolates were used to evaluate the accuracy of two selective growth media, four systems for biochemical identification (API 20NE, Vitek GNI, Vitek NFC, and MicroScan), and three different PCR-based assays. The PCR assays amplify different parts of the ribosomal DNA operon, either alone or in combination with cleavage by various restriction enzymes (PCR-restriction fragment length polymorphism [RFLP] analysis). The best system for the biochemical identification of B. cepacia appeared to be the API 20NE test. None of the biochemical assays successfully grouped the B. gladioli strains. The PCR-RFLP method appeared to be the optimal method for accurate nucleic acid-mediated identification of the different Burkholderia spp. With this method, B. gladioli was also reliably classified in a separate group. For the laboratory diagnosis of B. cepacia, we recommend parallel cultures on blood agar medium and selective agar plates. Further identification of colonies with a Burkholderia phenotype should be performed with the API 20NE test. For final confirmation of species identities, PCR amplification of the small-subunit rRNA gene followed by RFLP analysis with various enzymes is recommended.
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