ArticlePlant physiology1999
Heterologous expression of a plant small heat-shock protein enhances Escherichia coli viability under heat and cold stress.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 144 citations in OpenAlex.
- Toward a systems metabolic engineering approach to improve thermotolerance of Escherichia coli.Microbial cell factories · 2026Review
- Effects of small heat shock proteins from thermotolerant bacteria on the stress resistance of Escherichia coli to temperature, pH, and hyperosmolarity.Extremophiles : life under extreme conditions · 2024Article
- Polyextremophile engineering: a review of organisms that push the limits of life.Frontiers in microbiology · 2024Review
- Comparative Physiological and Transcriptome Analyses of Tolerant and Susceptible Cultivars Reveal the Molecular Mechanism of Cold Tolerance inInternational journal of molecular sciences · 2023Article
- European and American chestnuts: An overview of the main threats and control efforts.Frontiers in plant science · 2022Review
- A class I cytosolic HSP20 of rice enhances heat and salt tolerance in different organisms.Scientific reports · 2020Article
- The Mitochondrial Small Heat Shock Protein HSP22 from Pea is a Thermosoluble Chaperone Prone to Co-Precipitate with Unfolding Client Proteins.International journal of molecular sciences · 2019Article
- Improving stress tolerance and cell integrity of Rhodococcus ruber by overexpressing small-shock-protein Hsp16 of Rhodococcus.Journal of industrial microbiology & biotechnology · 2018Article
- A Class II small heat shock protein OsHsp18.0 plays positive roles in both biotic and abiotic defense responses in rice.Scientific reports · 2017Article
- Expression of CeHSP17 Protein in Response to Heat Shock and Heavy Metal Ions.Journal of nematology · 2017Article
- A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σScientific reports · 2017Article
- LEA proteins are involved in cyst desiccation resistance and other abiotic stresses in Azotobacter vinelandii.Cell stress & chaperones · 2017Article
- The CsHSP17.2 molecular chaperone is essential for thermotolerance in Camellia sinensis.Scientific reports · 2017Article
- Drought and Heat Differentially Affect XTH Expression and XET Activity and Action in 3-Day-Old Seedlings of Durum Wheat Cultivars with Different Stress Susceptibility.Frontiers in plant science · 2016Article
- Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer.BMC genomics · 2015Article
- A novel mechanism for small heat shock proteins to function as molecular chaperones.Scientific reports · 2015Article
- A systematic exploration of high-temperature stress-responsive genes in potato using large-scale yeast functional screening.Molecular genetics and genomics : MGG · 2014Article
- Plantation forestry under global warming: hybrid poplars with improved thermotolerance provide new insights on the in vivo function of small heat shock protein chaperones.Plant physiology · 2014Article
- Article
- Isolation of salt stress-related genes from Aspergillus glaucus CCHA by random overexpression in Escherichia coli.TheScientificWorldJournal · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A small heat-shock protein (sHSP) that shows molecular chaperone activity in vitro was recently purified from mature chestnut (Castanea sativa) cotyledons. This protein, renamed here as CsHSP17. 5, belongs to cytosolic class I, as revealed by cDNA sequencing and immunoelectron microscopy. Recombinant CsHSP17.5 was overexpressed in Escherichia coli to study its possible function under stress conditions. Upon transfer from 37 degrees C to 50 degrees C, a temperature known to cause cell autolysis, those cells that accumulated CsHSP17.5 showed improved viability compared with control cultures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of cell lysates suggested that such a protective effect in vivo is due to the ability of recombinant sHSP to maintain soluble cytosolic proteins in their native conformation, with little substrate specificity. To test the recent hypothesis that sHSPs may be involved in protection against cold stress, we also studied the viability of recombinant cells at 4 degrees C. Unlike the major heat-induced chaperone, GroEL/ES, the chestnut sHSP significantly enhanced cell survivability at this temperature. CsHSP17.5 thus represents an example of a HSP capable of protecting cells against both thermal extremes. Consistent with these findings, high-level induction of homologous transcripts was observed in vegetative tissues of chestnut plantlets exposed to either type of thermal stress but not salt stress.
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