ArticlePlant physiology1999
Identification of a cis-regulatory element involved in phytochrome down-regulated expression of the pea small GTPase gene pra2.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 29 citations in OpenAlex.
- Characterization and Functional Implications of the Nonexpressor of Pathogenesis-Related Genes 1 (International journal of molecular sciences · 2022Article
- Establishing an efficient protoplast transient expression system for investigation of floral thermogenesis in aroids.Plant cell reports · 2022Article
- A comparative genomic and transcriptomic analysis at the level of isolated root hair cells reveals new conserved root hair regulatory elements.Plant molecular biology · 2017Article
- ARF1 and SAR1 GTPases in endomembrane trafficking in plants.International journal of molecular sciences · 2013Review
- Identification of promoter motifs involved in the network of phytochrome A-regulated gene expression by combined analysis of genomic sequence and microarray data.Plant physiology · 2003Article
- Root-specific expression of a western white pine PR10 gene is mediated by different promoter regions in transgenic tobacco.Plant molecular biology · 2003Article
- Phytochrome-hormonal signalling networks.The New phytologist · 2003Review
- A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences.Nucleic acids research · 2001Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fusion DNA of its 5'-upstream region in front of the gene for firefly luciferase. Using this construct in a transient assay, we determined a pra2 cis-regulatory region sufficient to direct the light down-regulation of the luciferase reporter gene. Both 5'- and internal deletion analyses revealed that the 93-bp sequence between -734 and -642 from the transcriptional start site was important for phytochrome down-regulation. Gain-of-function analysis showed that this 93-bp region could confer light down-regulation when fused to the cauliflower mosaic virus 35S promoter. Furthermore, linker-scanning analysis showed that a 12-bp sequence within the 93-bp region mediated phytochrome down-regulation. Gel-retardation analysis showed the presence of a nuclear factor that was specifically bound to the 12-bp sequence in vitro. These results indicate that this element is a cis-regulatory element involved in phytochrome down-regulated expression.
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