ArticleJournal of virology1999
Contributions of viral splice sites and cis-regulatory elements to lentivirus vector function.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- An optimized protocol for quality control of gene therapy vectors using nanopore direct RNA sequencing.Genome research · 2024Article
- Review
- Distinct chromatin functional states correlate with HIV latency reactivation in infected primary CD4eLife · 2018Article
- A Fourth Generation Lentiviral Vector: Simplifying Genomic Gymnastics.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Eliminating HIV-1 Packaging Sequences from Lentiviral Vector Proviruses Enhances Safety and Expedites Gene Transfer for Gene Therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- A doubly fluorescent HIV-1 reporter shows that the majority of integrated HIV-1 is latent shortly after infection.Journal of virology · 2013Article
- Biosafety features of lentiviral vectors.Human gene therapy · 2013Review
- Blocking double-stranded RNA-activated protein kinase PKR by Japanese encephalitis virus nonstructural protein 2A.Journal of virology · 2012Article
- Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag.Journal of virology · 2012Article
- Dengue virus targets the adaptor protein MITA to subvert host innate immunity.PLoS pathogens · 2012Article
- The HIV-1 Rev protein enhances encapsidation of unspliced and spliced, RRE-containing lentiviral vector RNA.PloS one · 2012Article
- Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repair.BMC biotechnology · 2009Article
- The cellular antiviral protein viperin is attenuated by proteasome-mediated protein degradation in Japanese encephalitis virus-infected cells.Journal of virology · 2008Article
- Flavivirus infection activates the XBP1 pathway of the unfolded protein response to cope with endoplasmic reticulum stress.Journal of virology · 2006Article
- Development of a human immunodeficiency virus vector-based, single-cycle assay for evaluation of anti-integrase compounds.Antimicrobial agents and chemotherapy · 2006Article
- Induction of primary anti-HIV CD4 and CD8 T cell responses by dendritic cells transduced with self-inactivating lentiviral vectors.Cellular immunology · 2006Article
- Blocking of interferon-induced Jak-Stat signaling by Japanese encephalitis virus NS5 through a protein tyrosine phosphatase-mediated mechanism.Journal of virology · 2006Article
- Theoretical design of a gene therapy to prevent AIDS but not human immunodeficiency virus type 1 infection.Journal of virology · 2003Article
- RNA 3' readthrough of oncoretrovirus and lentivirus: implications for vector safety and efficacy.Journal of virology · 2002Article
- Concerted action of multiple cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The mobile transgene constructs of most human immunodeficiency virus (HIV)-based lentivirus vectors currently in use contain viral long terminal repeats, a 5' untranslated region, gag sequences, and env sequences that include the Rev-responsive element (RRE). In this study, we examined the possibility of deleting HIV splice sites and gag and env sequences from an HIV type 1 recombinant vector established in our laboratory as part of our ongoing efforts to improve this vector system. Mutations in the major splice donor site (SD) markedly reduced viral RNA expression but had little effect on vector titer. Deletion of gag or env sequences, excluding RRE, led to a moderate reduction in vector titer. Interestingly, deletion of RRE slightly reduced viral RNA expression but markedly impaired vector function. Combined deletions of RRE, gag (except for the first 40 nucleotides), env, and the SD mutation resulted in a twofold increase in cytoplasmic viral RNA expression and a recovery of vector efficiency to approximately 50% of the wild-type level. This increase in cytoplasmic RNA levels is likely to be due, at least in part, to effects of the TE671 host cells, a human rhabdomyosarcoma cell line used for vector production in our system, on the cytoplasmic distribution of spliced and unspliced viral RNA. These results show that optimal lentivirus vector function can be maintained in the absence of multiple essential viral elements.
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Registered trials
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