ArticleJournal of virology1999
Amelioration of retroviral vector silencing in locus control region beta-globin-transgenic mice and transduced F9 embryonic cells.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Characterization of Gene Alterations following Editing of the β-Globin Gene Locus in Hematopoietic Stem/Progenitor Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- miR-196b directly targets both HOXA9/MEIS1 oncogenes and FAS tumour suppressor in MLL-rearranged leukaemia.Nature communications · 2012Article
- Article
- Rapid transcriptional pulsing dynamics of high expressing retroviral transgenes in embryonic stem cells.PloS one · 2012Article
- Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse.PloS one · 2011Article
- Expression of Fbxo7 in haematopoietic progenitor cells cooperates with p53 loss to promote lymphomagenesis.PloS one · 2011Article
- Article
- MECP2 isoform-specific vectors with regulated expression for Rett syndrome gene therapy.PloS one · 2009Article
- EOS lentiviral vector selection system for human induced pluripotent stem cells.Nature protocols · 2009Article
- Chromatin, gene silencing and HIV latency.Genome biology · 2007Review
- Stable gammaretroviral vector expression during embryonic stem cell-derived in vitro hematopoietic development.Molecular therapy : the journal of the American Society of Gene Therapy · 2006Article
- Retrovirus silencer blocking by the cHS4 insulator is CTCF independent.Nucleic acids research · 2003Article
- The Moloney murine leukemia virus repressor binding site represses expression in murine and human hematopoietic stem cells.Journal of virology · 2003Article
- Efficient marking of neural stem cell-derived neurons with a modified murine embryonic stem cell virus, MESV2.Gene therapy · 2002Article
- Enhanced transgene expression in primitive hematopoietic progenitor cells and embryonic stem cells efficiently transduced by optimized retroviral hybrid vectors.Gene therapy · 2002Article
- A high-capacity, capsid-modified hybrid adenovirus/adeno-associated virus vector for stable transduction of human hematopoietic cells.Journal of virology · 2002Article
- Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Genomic targeting of methylated DNA: influence of methylation on transcription, replication, chromatin structure, and histone acetylation.Molecular and cellular biology · 2000Article
- Retrovirus vector silencing is de novo methylase independent and marked by a repressive histone code.The EMBO journal · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Retroviral vectors are transcriptionally silenced in hematopoietic stem cells, and this phenomenon must be overcome for effective gene therapy of blood diseases. The murine stem cell virus (MSCV) vector completely silences beta-globin reporter genes regulated by locus control region (LCR) elements 5'HS2 to 5'HS4 in seven of eight transgenic mice. Here, we show that no single known MSCV silencer element is sufficient for complete LCR beta-globin transgene silencing. However, partial silencing of high-copy transgenes is conveyed by the MSCV direct repeat and promoter elements. The CpG methylation pattern of silenced and expressed MSCV promoter transgenes is virtually identical, demonstrating that silencing does not absolutely correlate with methylation status. Combined mutations in all four MSCV silencer elements leads to expression of beta-globin in 6 of 10 transgenic mice. The same mutations incorporated into the HSC1 retrovirus vector direct neo gene expression in 71% of transduced F9 embryonic carcinoma cells. These studies demonstrate that combined mutation of four retroviral silencer elements relieves complete silencing in most transgenic mice and transduced F9 cells and suggests that novel silencer elements remain. Enhanced expression of the HSC1 vector in primitive stem cells is well suited for blood gene therapy applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.