ArticleBritish journal of cancer1999
Amphiregulin acts as an autocrine growth factor in two human polarizing colon cancer lines that exhibit domain selective EGF receptor mitogenesis.
Article in British journal of cancer, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- Cadherins and catenins in cancer: connecting cancer pathways and tumor microenvironment.Frontiers in cell and developmental biology · 2023Review
- Recent Advances in the Study of Extracellular Vesicles in Colorectal Cancer.Gastroenterology · 2022Review
- Loss of miR-24-3p promotes epithelial cell apoptosis and impairs the recovery from intestinal inflammation.Cell death & disease · 2021Article
- Article
- Amphiregulin enhances intercellular adhesion molecule-1 expression and promotes tumor metastasis in human osteosarcoma.Oncotarget · 2015Article
- In vitro combined treatment with cetuximab and trastuzumab inhibits growth of colon cancer cells.Cell proliferation · 2014Article
- Trafficking of epidermal growth factor receptor ligands in polarized epithelial cells.Annual review of physiology · 2014Review
- Identification of the angiogenic gene signature induced by EGF and hypoxia in colorectal cancer.BMC cancer · 2013Article
- Effect of blockade of the EGF system on wound healing in patients vaccinated with CIMAvax® EGF.World journal of surgical oncology · 2013Article
- Myofibroblast keratinocyte growth factor reduces tight junctional integrity and increases claudin-2 levels in polarized Caco-2 cells.Growth factors (Chur, Switzerland) · 2012Article
- Ret heterozygous mice have enhanced intestinal adaptation after massive small bowel resection.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Nuclear translocation of pro-amphiregulin induces chemoresistance in gastric cancer.Cancer science · 2012Article
- Identification of a novel mono-leucine basolateral sorting motif within the cytoplasmic domain of amphiregulin.Traffic (Copenhagen, Denmark) · 2011Article
- Oncogenic KRAS desensitizes colorectal tumor cells to epidermal growth factor receptor inhibition and activation.Neoplasia (New York, N.Y.) · 2010Article
- The role of cyclooxygenase-2 in cell proliferation and cell death in human malignancies.International journal of cell biology · 2010Article
- Article
- Bile acid alone, or in combination with acid, induces CDX2 expression through activation of the epidermal growth factor receptor (EGFR).Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2009Article
- Article
- Amphiregulin as a novel target for breast cancer therapy.Journal of mammary gland biology and neoplasia · 2008Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Colonic enterocytes, like many epithelial cells in vivo, are polarized with functionally distinct apical and basolateral membrane domains. The aims of this study were to characterize the endogenous epidermal growth factor (EGF)-like ligands expressed in two polarizing colon cancer cell lines, HCA-7 Colony 29 (HCA-7) and Caco-2, and to examine the effects of cell polarity on EGF receptor-mediated mitogenesis. HCA-7 and Caco-2 cells were grown on plastic, or as a polarized monolayer on Transwell filters. Cell proliferation was measured by 3H-thymidine incorporation and EGF receptor (EGFR) binding was assessed by Scatchard analysis. EGFR ligand expression was determined by Northern blot analysis, reverse transcription polymerase chain reaction, metabolic labelling and confocal microscopy. We found that amphiregulin (AR) was the most abundant EGFR ligand expressed in HCA-7 and Caco-2 cells. AR was localized to the basolateral surface and detected in basolateral-conditioned medium. Basolateral administration of neutralizing AR antibodies significantly reduced basal DNA replication. A single class of high-affinity EGFRs was detected in the basolateral compartment, whereas the apical compartment of polarized cells, and cells cultured on plastic, displayed two classes of receptor affinity. Basolateral administration of transforming growth factor alpha (TGF-alpha) or an EGFR neutralizing antibody also resulted in a dose-dependent stimulation or attenuation, respectively, of DNA replication. However, no mitogenic response was observed when these agents were added to the apical compartment or to confluent cells cultured on plastic. We conclude that amphiregulin acts as an autocrine growth factor in HCA-7 and Caco-2 cells, and EGFR ligand-induced proliferation is influenced by cellular polarity.
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