ArticleProceedings of the National Academy of Sciences of the United States of America1999
Recombinant Semliki Forest virus and Sindbis virus efficiently infect neurons in hippocampal slice cultures.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 184 citations in OpenAlex.
- Engineering a streamlined virus-like particle for programmable tissue-specific gene delivery.Nature communications · 2025Article
- Packaging, Purification, and Titration of Replication-Deficient Semliki Forest Virus-Derived Particles as a Self-Amplifying mRNA Vaccine VectorIranian biomedical journal · 2022Article
- Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants.Viruses · 2020Article
- Review
- Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution.National science review · 2019Article
- Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus.Neuroscience bulletin · 2019Article
- The Effects of Sindbis Viral Vectors on Neuronal Function.Frontiers in cellular neuroscience · 2019Article
- Optogenetic manipulation of medullary neurons in the locust optic lobe.Journal of neurophysiology · 2018Article
- Detection of phasic dopamine by D1 and D2 striatal medium spiny neurons.The Journal of physiology · 2017Article
- Viral vectors for therapy of neurologic diseases.Neuropharmacology · 2017Review
- PTEN recruitment controls synaptic and cognitive function in Alzheimer's models.Nature neuroscience · 2016Article
- A New Defective Helper RNA to Produce Recombinant Sindbis Virus that Infects Neurons but does not Propagate.Frontiers in neuroanatomy · 2016Article
- Alphaviruses in gene therapy.Viruses · 2015Review
- LTP-triggered cholesterol redistribution activates Cdc42 and drives AMPA receptor synaptic delivery.The Journal of cell biology · 2015Article
- Neuroanatomy goes viral!Frontiers in neuroanatomy · 2015Review
- Alphavirus-based vaccines.Viruses · 2014Review
- Striatal neurones have a specific ability to respond to phasic dopamine release.The Journal of physiology · 2013Article
- The NO/cGMP pathway inhibits transient cAMP signals through the activation of PDE2 in striatal neurons.Frontiers in cellular neuroscience · 2013Article
- Analysis of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain.PloS one · 2013Article
- Reprogramming axonal behavior by axon-specific viral transduction.Gene therapy · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene transfer into nervous tissue is a powerful tool for the analysis of gene function. By using a rat hippocampal slice culture preparation, we show here that Semliki Forest virus (SFV) and Sindbis virus (SIN) vectors are useful for the effective infection of neurons. The stratum pyramidale and/or the granular cell layer were injected with recombinant virus encoding beta-galactosidase (LacZ) or green fluorescent protein (GFP). By using low concentrations of injected SFV-LacZ or SIN-LacZ, we detected LacZ staining of pyramidal cells, interneurons, and granule cells. About 60% of the infected cells showed clear neuronal morphology; thus, relatively few glial cells expressed the transgene. Expression of GFP from SFV and SIN vectors gave similar results, with an even higher percentage (>90%) of the GFP-positive cells identified as neurons. Infected pyramidal cells were readily recognized in living slices, displaying GFP fluorescence in dendrites of up to fourth order and in dendritic spines. They appeared morphologically normal and viable at 1-5 days postinfection. We conclude that both SFV and SIN vectors efficiently transfer genes into neurons in hippocampal slice cultures. In combination with the GFP reporter, SFV and SIN vectors will allow the physiological examination of identified neurons that have been modified by overexpression or suppression of a specific gene product.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.