ArticleProceedings of the National Academy of Sciences of the United States of America1999
Unexpected abundance of self-splicing introns in the genome of bacteriophage Twort: introns in multiple genes, a single gene with three introns, and exon skipping by group I ribozymes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 79 citations in OpenAlex.
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- Programming RNA Trans-Splicing for Versatile Gene Regulation and Complex Cellular Logic Computation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Self-splicing introns in genes of Bastillevirinae bacteriophages.Nucleic acids research · 2025Article
- An intron endonuclease facilitates interference competition between coinfecting viruses.Science (New York, N.Y.) · 2024Article
- Development of advanced control material for reverse transcription-mediated bacterial nucleic acid amplification tests.Journal of clinical microbiology · 2024Article
- Group I Intron as a Potential Target for Antifungal Compounds: Development of aMolecules (Basel, Switzerland) · 2023Article
- Pairwise Engineering of Tandemly Aligned Self-Splicing Group I Introns for Analysis and Control of Their Alternative Splicing.Biomolecules · 2023Article
- Pectobacterium Phage Jarilo Displays Broad Host Range and Represents a Novel Genus of Bacteriophages Within the FamilyPHAGE (New Rochelle, N.Y.) · 2020Article
- Expanding the Diversity ofViruses · 2019Article
- Characterization of vB_SauM-fRuSau02, a Twort-Like Bacteriophage Isolated from a Therapeutic Phage Cocktail.Viruses · 2017Article
- Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains.PloS one · 2017Article
- The genome of AR9, a giant transducing Bacillus phage encoding two multisubunit RNA polymerases.Virology · 2016Article
- Article
- Single-Cell (Meta-)Genomics of a Dimorphic Candidatus Thiomargarita nelsonii Reveals Genomic Plasticity.Frontiers in microbiology · 2016Article
- Genome organisation of the Acinetobacter lytic phage ZZ1 and comparison with other T4-like Acinetobacter phages.BMC genomics · 2014Article
- Romulus and Remus, two phage isolates representing a distinct clade within the Twortlikevirus genus, display suitable properties for phage therapy applications.Journal of virology · 2013Article
- Complete genome sequence of Staphylococcus aureus bacteriophage GH15.Journal of virology · 2012Article
- Ribozyme stability, exon skipping, and a potential role for RNA helicase in group I intron splicing by Coxiella burnetii.Journal of bacteriology · 2011Article
- RNA molecules with conserved catalytic cores but variable peripheries fold along unique energetically optimized pathways.RNA (New York, N.Y.) · 2011Article
- T4-Like genome organization of the Escherichia coli O157:H7 lytic phage AR1.Journal of virology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Analysis of RNA that can be labeled with GTP indicates the existence of group I introns in genes of at least three transcriptional classes in the genome of Staphylococcus aureus bacteriophage Twort. A single ORF of 142 amino acids (Orf142) is interrupted by three self-splicing group I introns, providing the first example of a phage gene with multiple intron insertions. Twort Orf142 is encoded in a message that is abundant 15-20 min after infection and is highly similar to a late gene product (Orf8) of the morphologically related Listeria phage A511. The introns in orf142 are spliced in vivo and contain all the conserved features of primary sequence and secondary structure of group I introns in subgroup IA2, which includes the introns in Escherichia coli phage T4 and the Bacillus phages beta22 and SPO1. Introns I2 and I3 in orf142 are highly similar, and their intron insertion sites are closely spaced. The presence of transcripts with a skipped exon between these introns indicates that they may fold into a single active ribozyme resulting in alternative splicing. Alternatively, the cleaved 5' exon preceding I2 may undergo trans splicing to the 3' exon that follows I3. Regardless of the detailed mechanism, these results demonstrate a new means whereby a single gene can give rise to multiple messenger RNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.