ArticleProceedings of the National Academy of Sciences of the United States of America1999
Phage-display library selection of high-affinity human single-chain antibodies to tumor-associated carbohydrate antigens sialyl Lewisx and Lewisx.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 94 citations in OpenAlex.
- Trial
- Editor's Choice Protein engineering strategies to develop lectins by design.Glycobiology · 2025Review
- Phage Display Isolation of VHH Antibodies Targeting Capsular Polysaccharides of Intratumor Bacteria.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Smaller size packs a stronger punch - Recent advances in small antibody fragments targeting tumour-associated carbohydrate antigens.Theranostics · 2023Review
- Altered Glycan Expression on Breast Cancer Cells Facilitates Infection by T3 Seroptype Oncolytic Reovirus.Nano letters · 2021Article
- Increasing the Affinity of an O-Antigen Polysaccharide Binding Site in Shigella flexneri Bacteriophage Sf6 Tailspike Protein.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Advances in the Production and Batch Reformatting of Phage Antibody Libraries.Molecular biotechnology · 2019Review
- Generation of a Fully Human scFv that binds Tumor-Specific Glycoforms.Scientific reports · 2019Article
- A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity.Scientific reports · 2017Article
- The diagnostic targeting of a carbohydrate virulence factor from M.Tuberculosis.Scientific reports · 2015Article
- Production of a recombinant antibody specific for i blood group antigen, a mesenchymal stem cell marker.BioResearch open access · 2013Article
- Isolation of human monoclonal antibodies from peripheral blood B cells.Nature protocols · 2013Article
- Phage display-based strategies for cloning and optimization of monoclonal antibodies directed against human pathogens.International journal of molecular sciences · 2012Review
- Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein.Biotechnology research international · 2011Article
- Spatially addressed combinatorial protein libraries for recombinant antibody discovery and optimization.Nature biotechnology · 2010Article
- Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression.The Journal of biological chemistry · 2010Article
- Targeting activated integrin alphavbeta3 with patient-derived antibodies impacts late-stage multiorgan metastasis.Clinical & experimental metastasis · 2010Article
- Inhibition of tumor metastasis: functional immune modulation of the CUB domain containing protein 1.Molecular pharmaceutics · 2010Article
- Phage display on the base of filamentous bacteriophages: application for recombinant antibodies selection.Acta naturae · 2009Article
- Selection and characterization of a human monoclonal neutralizing antibody for Clostridium Botulinum neurotoxin serotype B.Bioorganic & medicinal chemistry letters · 2009Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
mAbs against tumor-associated carbohydrate antigens have the potential to play a prominent role in cancer immunotherapy. However, it has not been possible to fully exploit the clinical utility of such antibodies primarily, because those of adequate affinity could be derived only from murine sources. To address this problem, we prepared a single-chain Fv (scFv) antibody library from the peripheral blood lymphocytes of 20 patients with various cancer diseases. Completely human high-affinity scFv antibodies were then selected by using synthetic sialyl Lewisx and Lewisx BSA conjugates. These human scFv antibodies were specific for sialyl Lewisx and Lewisx, as demonstrated by ELISA, BIAcore, and flow cytometry binding to the cell surface of pancreatic adenocarcinoma cells. Nucleotide sequencing revealed that at least four unique scFv genes were obtained. The Kd values ranged from 1.1 to 6.2 x 10(-7) M that were comparable to the affinities of mAbs derived from the secondary immune response. These antibodies could be valuable reagents for probing the structure and function of carbohydrate antigens and in the treatment of human tumor diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.