ArticleProceedings of the National Academy of Sciences of the United States of America1999
An experimental solution for the Luria-Delbrück fluctuation problem in measuring hypermutation rates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- A Framework for Investigating Rules of Life by Establishing Zones of Influence.Integrative and comparative biology · 2022Article
- Proliferation model dependence in fluctuation analysis: the neutral case.Journal of mathematical biology · 2010Article
- A quantitative analysis of genomic instability in lymphoid and plasma cell neoplasms based on the PIG-A gene.Mutation research · 2010Article
- The rate of mutation of a single gene.Nucleic acids research · 2010Article
- Methods for determining spontaneous mutation rates.Methods in enzymology · 2006Article
- AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of nuclear localization.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Reduced rates of gene loss, gene silencing, and gene mutation in Dnmt1-deficient embryonic stem cells.Molecular and cellular biology · 2001Article
- Memories of a mentor: Charley Steinberg.Genetics · 2001Article
- Sorting out mutation rates.Proceedings of the National Academy of Sciences of the United States of America · 1999Review
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5 authors at 3 institutions in 3 countries.
Funding
Abstract
A cell line harboring all trans-acting elements necessary for hypermutation was transfected with a plasmid harboring the major cis-acting elements plus a green fluorescent protein gene containing a premature chain-termination codon. Transfected cells do not fluoresce unless the stop codon reverts. When a sizable cell population is purged of revertants by sorting, the frequency of mutants increases linearly with time, and there is no Luria-Delbrück fluctuation effect. Moreover, as mutant frequencies seemed to vary less than cell numbers in replicate cultures, it is suggested that hypermutation might not be coupled closely to cell division.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.