ArticleProceedings of the National Academy of Sciences of the United States of America1999
Two distinct forms of the 64,000 Mr protein of the cleavage stimulation factor are expressed in mouse male germ cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Identification and Functional Analysis of ThreeInsects · 2024Article
- Post-transcriptional regulation in spermatogenesis: all RNA pathways lead to healthy sperm.Cellular and molecular life sciences : CMLS · 2021Review
- A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans.Nucleic acids research · 2020Article
- Connections between 3' end processing and DNA damage response: Ten years later.Wiley interdisciplinary reviews. RNA · 2020Review
- Alternative cleavage and polyadenylation in health and disease.Nature reviews. Genetics · 2019Review
- Tissue-specific mechanisms of alternative polyadenylation: Testis, brain, and beyond (2018 update).Wiley interdisciplinary reviews. RNA · 2019Review
- Genome-wide atlas of alternative polyadenylation in the forage legume red clover.Scientific reports · 2018Article
- Cstf2t Regulates expression of histones and histone-like proteins in male germ cells.Andrology · 2018Article
- TauCstF-64 Mediates Correct mRNA Polyadenylation and Splicing of Activator and Repressor Isoforms of the Cyclic AMP-Responsive Element Modulator (CREM) in Mouse Testis.Biology of reproduction · 2016Article
- The Cstf2t Polyadenylation Gene Plays a Sex-Specific Role in Learning Behaviors in Mice.PloS one · 2016Article
- The end of the message: multiple protein-RNA interactions define the mRNA polyadenylation site.Genes & development · 2015Review
- Generation of plasmid vectors expressing FLAG-tagged proteins under the regulation of human elongation factor-1α promoter using Gibson assembly.Journal of visualized experiments : JoVE · 2015Article
- Comparative RNA-Seq analysis reveals pervasive tissue-specific alternative polyadenylation in Caenorhabditis elegans intestine and muscles.BMC biology · 2015Article
- CstF-64 is necessary for endoderm differentiation resulting in cardiomyocyte defects.Stem cell research · 2014Article
- New links between mRNA polyadenylation and diverse nuclear pathways.Molecules and cells · 2014Review
- CstF-64 supports pluripotency and regulates cell cycle progression in embryonic stem cells through histone 3' end processing.Nucleic acids research · 2014Article
- Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.Molecular and cellular biology · 2014Review
- High-throughput sequencing of RNA isolated by cross-linking and immunoprecipitation (HITS-CLIP) to determine sites of binding of CstF-64 on nascent RNAs.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Overlapping and distinct functions of CstF64 and CstF64τ in mammalian mRNA 3' processing.RNA (New York, N.Y.) · 2013Article
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8 authors.
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Abstract
Polyadenylation in male germ cells differs from that in somatic cells. Many germ cell mRNAs do not contain the canonical AAUAAA in their 3' ends but are efficiently polyadenylated. To determine whether the 64,000 Mr protein of the cleavage stimulation factor (CstF-64) is altered in male germ cells, we examined its expression in mouse testis. In addition to the 64,000 Mr form, we found a related approximately 70,000 Mr protein that is abundant in testis, at low levels in brain, and undetectable in all other tissues examined. Expression of the approximately 70,000 Mr CstF-64 was limited to meiotic spermatocytes and postmeiotic spermatids in testis. In contrast, the 64,000 Mr form was absent from spermatocytes, suggesting that the testis-specific CstF-64 might control expression of meiosis-specific genes. To determine why the 64,000 Mr CstF-64 is not expressed in spermatocytes, we mapped its chromosomal location to the X chromosome in both mouse and human. CstF-64 may, therefore, be absent in spermatocytes because the X chromosome is inactivated during male meiosis. By extension, the testis-specific CstF-64 may be expressed from an autosomal homolog of the X chromosomal gene.
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