ArticleProceedings of the National Academy of Sciences of the United States of America1999
Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 230 citations in OpenAlex.
- The Catsper channel and its roles in male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2017Pooled it
- Zinc is a key regulator of the sperm-specific KeLife · 2026Article
- Sperm plasma membrane potential as a marker for fertilization success for non-normozoospermic patients undergoing assisted reproduction technologies.Journal of assisted reproduction and genetics · 2026Article
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- Membrane potential and feedback dynamics regulate CatSper-mediated progesterone signaling in human sperm.bioRxiv : the preprint server for biology · 2025Article
- Signaling Roleplay between Ion Channels during Mammalian Sperm Capacitation.Biomedicines · 2023Review
- SLO3: A Conserved Regulator of Sperm Membrane Potential.International journal of molecular sciences · 2023Review
- CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Membrane-Mediated Regulation of Sperm Fertilization Potential in Poultry.The journal of poultry science · 2022Review
- Capacitation promotes a shift in energy metabolism in murine sperm.Frontiers in cell and developmental biology · 2022Article
- A Minimal Model Shows that a Positive Feedback Loop Between sNHE and SLO3 can Control Mouse Sperm Capacitation.Frontiers in cell and developmental biology · 2022Article
- Article
- Molecular Mechanism Underlying the Action ofFrontiers in cell and developmental biology · 2020Article
- Membrane Potential Assessment by Fluorimetry as a Predictor Tool of Human Sperm Fertilizing Capacity.Frontiers in cell and developmental biology · 2019Article
- Determination of a Robust Assay for Human Sperm Membrane Potential Analysis.Frontiers in cell and developmental biology · 2019Article
- Molecular Basis of Human Sperm Capacitation.Frontiers in cell and developmental biology · 2018Review
- Gamete activation: basic knowledge and clinical applications.Human reproduction update · 2016Review
- Flow cytometry analysis reveals that only a subpopulation of mouse sperm undergoes hyperpolarization during capacitation.Biology of reproduction · 2015Article
- SLO3 K+ channels control calcium entry through CATSPER channels in sperm.The Journal of biological chemistry · 2014Article
- Membrane hyperpolarization during human sperm capacitation.Molecular human reproduction · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Sperm adhesion to egg zonae pellucidae initiates sperm acrosome reactions, an exocytotic event that is an early step during fertilization. Previously, it was suggested that zona pellucida-evoked Ca2+ entry into sperm through low voltage-activated Ca2+ channels is an essential step in acrosome reactions, based on the inhibitory effects of Ca2+ channel antagonists. However, analysis of this channel is limited by the inability to apply electrophysiological methods directly to sperm. In this report, optical methods of determining membrane potential and internal Ca2+ levels were used to demonstrate that (i) contact with zonae pellucidae activates a transient Ca2+ response in sperm that has a time course and antagonist sensitivity anticipated of low voltage-activated Ca2+ channels; (ii) these channels are unavailable for opening in uncapacitated sperm because of voltage-dependent, steady state inactivation; (iii) membrane hyperpolarization during sperm capacitation is sufficient to recruit channels into a closed state, from which they are available for opening during fertilization; and (iv) channel conductance state may be a factor in determines the efficacy with which channel antagonists inhibit fertilization. This study provides evidence for the activation of sperm Ca2+ channels during gamete adhesion and offers a mechanism that may account for aspects of the regulation of sperm fertility during capacitation through the control of channel availability. Finally, these results suggest that channel conductance state may be a central feature in the design of channel antagonists that inhibit sperm function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.