ArticleProceedings of the National Academy of Sciences of the United States of America1999
Signaling via beta1 integrins and mitogen-activated protein kinase determines human epidermal stem cell fate in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed.
- Double-sided niche regulation in skin stem cell and cancer: mechanisms and clinical applications.Molecular cancer · 2025Review
- Integrins control epithelial stem cell proliferation in theFrontiers in cell and developmental biology · 2023Article
- The proliferative and multipotent epidermal progenitor cells for human skin reconstruction in vitro and in vivo.Cell proliferation · 2022Article
- Cutis verticis gyrata and Noonan syndrome: report of two cases with pathogenetic variant in SOS1 gene.Italian journal of pediatrics · 2022Article
- Apoptosis and tissue thinning contribute to symmetric cell division in the developing mouse epidermis in a nonautonomous way.PLoS biology · 2022Article
- Skin Minerals: Key Roles of Inorganic Elements in Skin Physiological Functions.International journal of molecular sciences · 2022Review
- Epidermal Basement Membrane Substitutes for Bioengineering of Human Epidermal Equivalents.JID innovations : skin science from molecules to population health · 2022Article
- Plating human iPSC lines on micropatterned substrates reveals role for ITGB1 nsSNV in endoderm formation.Stem cell reports · 2021Article
- Article
- GDF-5 promotes epidermal stem cells proliferation via Foxg1-cyclin D1 signaling.Stem cell research & therapy · 2021Article
- Conventional and Emerging Markers in Stem Cell Isolation and Characterization.Advances in experimental medicine and biology · 2021Review
- Antioxidants as an Epidermal Stem Cell Activator.Antioxidants (Basel, Switzerland) · 2020Review
- Regulation of ERK basal and pulsatile activity control proliferation and exit from the stem cell compartment in mammalian epidermis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Flexibility sustains epithelial tissue homeostasis.Current opinion in cell biology · 2019Review
- Peptide-Functionalized Hydrogels Modulate Integrin Expression and Stemness in Adult Human Epidermal Keratinocytes.Advanced biosystems · 2019Article
- Phage-Based Artificial Niche: The Recent Progress and Future Opportunities in Stem Cell Therapy.Stem cells international · 2019Review
- Conditional ablation of p130Cas/BCAR1 adaptor protein impairs epidermal homeostasis by altering cell adhesion and differentiation.Cell communication and signaling : CCS · 2018Article
- Mechanical forces direct stem cell behaviour in development and regeneration.Nature reviews. Molecular cell biology · 2017Review
- Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics.Stem cells international · 2017Review
- Human keratinocytes have two interconvertible modes of proliferation.Nature cell biology · 2016Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human epidermal stem cells express higher levels of beta1 integrins and are more adhesive than keratinocytes that are destined to differentiate. To investigate whether high beta1 integrin expression and adhesiveness are essential for maintaining keratinocytes in the stem cell compartment, we introduced a dominant-negative beta1 integrin mutant, CD8beta1, into cultured human keratinocytes, thereby interfering with beta1 integrin function. Surface beta1 integrin levels, adhesiveness, and mitogen-activated protein (MAP) kinase activation on fibronectin were reduced, and exit from the stem cell compartment was stimulated. Adhesiveness and proliferative potential were restored by overexpressing wild-type beta1 integrin or by constitutive MAP kinase activation. Conversely, a dominant-negative MAP kinase kinase 1 mutant decreased adhesiveness and stem cell number in the absence of CD8beta1. MAP kinase activation by alpha6beta4-mediated adhesion and mitogens was normal in CD8beta1 cells, and constitutive MAP kinase activation did not affect adhesion and proliferation of control keratinocytes. We conclude that beta1 integrins and MAP kinase cooperate to maintain the epidermal stem cell compartment in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.