ArticleProceedings of the National Academy of Sciences of the United States of America1999
A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Aptamers, Riboswitches, and Ribozymes inLife (Basel, Switzerland) · 2021Review
- Disruption of dengue virus transmission by mosquitoes.Current opinion in insect science · 2015Article
- Co-transcriptional RNA cleavage provides a failsafe termination mechanism for yeast RNA polymerase I.Nucleic acids research · 2011Article
- Article
- Direct selection for ribozyme cleavage activity in cells.RNA (New York, N.Y.) · 2009Article
- Methodological obstacles in knocking down small noncoding RNAs.RNA (New York, N.Y.) · 2009Article
- Fast ribozyme cleavage releases transcripts from RNA polymerase II and aborts co-transcriptional pre-mRNA processing.Nature structural & molecular biology · 2009Article
- Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae.Genes & development · 2009Article
- Modulating alternative splicing by cotranscriptional cleavage of nascent intronic RNA.RNA (New York, N.Y.) · 2008Article
- A new connection of mRNP biogenesis and export with transcription-coupled repair.Nucleic acids research · 2007Article
- In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants.Genes & development · 2006Article
- Replication fork progression is impaired by transcription in hyperrecombinant yeast cells lacking a functional THO complex.Molecular and cellular biology · 2006Article
- A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript.RNA (New York, N.Y.) · 2004Article
- Kinetic analysis of ribozyme-substrate complex formation in yeast.RNA (New York, N.Y.) · 2004Article
- Differential subnuclear localization of RNA strands of opposite polarity derived from an autonomously replicating viroid.The Plant cell · 2003Article
- Polyadenylation of rRNA- and tRNA-based yeast transcripts cleaved by internal ribozyme activity.Current genetics · 2003Article
- A quantitative analysis of intron effects on mammalian gene expression.RNA (New York, N.Y.) · 2003Article
- The stable 2.0-kilobase intron of the herpes simplex virus type 1 latency-associated transcript does not function as an antisense repressor of ICP0 in nonneuronal cells.Journal of virology · 2003Article
- Interactions between mRNA export commitment, 3'-end quality control, and nuclear degradation.Molecular and cellular biology · 2002Article
- Small nucleolar RNAs: versatile trans-acting molecules of ancient evolutionary origin.Gene expression · 2002Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a "snorbozyme," is metabolically stable and cleaves a target U3 RNA with nearly 100% efficiency in vivo. This is the most efficient in vivo cleavage reported for a trans-acting ribozyme. A key advantage of the model substrate featured is that a stable, trimmed cleavage product accumulates. This property allows accurate kinetic measurements of authentic cleavage in vivo. The system offers new avenues for developing effective ribozymes for research and therapeutic applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.