ArticleThe Biochemical journal1999
N-terminal domains of fibrillin 1 and fibrillin 2 direct the formation of homodimers: a possible first step in microfibril assembly.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- The fibrillinopathies: New insights with focus on the paradigm of opposing phenotypes for both FBN1 and FBN2.Human mutation · 2022Review
- Outcomes of Iris-Claw IOL Implantation in Patients with Marfan's Syndrome in Jordan.Clinical ophthalmology (Auckland, N.Z.) · 2022Article
- Identification of the growth factor-binding sequence in the extracellular matrix protein MAGP-1.The Journal of biological chemistry · 2020Article
- The role of fibrillin and microfibril binding proteins in elastin and elastic fibre assembly.Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- Fell-Muir Lecture: Fibrillin microfibrils: structural tensometers of elastic tissues?International journal of experimental pathology · 2017Review
- Fibrillin-containing microfibrils are key signal relay stations for cell function.Journal of cell communication and signaling · 2015Article
- Molecular pathogenesis and management strategies of ectopia lentis.Eye (London, England) · 2014Review
- The organisation of elastin and fibrillins 1 and 2 in the cruciate ligament complex.Journal of anatomy · 2011Article
- ADAMTSL-6 is a novel extracellular matrix protein that binds to fibrillin-1 and promotes fibrillin-1 fibril formation.The Journal of biological chemistry · 2010Article
- Biogenesis and function of fibrillin assemblies.Cell and tissue research · 2010Review
- Fibrillin assembly requires fibronectin.Molecular biology of the cell · 2009Article
- Biogenesis of extracellular microfibrils: Multimerization of the fibrillin-1 C terminus into bead-like structures enables self-assembly.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Extracellular matrix in the trabecular meshwork.Experimental eye research · 2008Review
- The molecular genetics of Marfan syndrome and related disorders.Journal of medical genetics · 2006Review
- Evidence for a critical contribution of haploinsufficiency in the complex pathogenesis of Marfan syndrome.The Journal of clinical investigation · 2004Article
- Fibrillin-1 and -2 contain heparin-binding sites important for matrix deposition and that support cell attachment.The Biochemical journal · 2003Article
- Identification of FBN1 gene mutations in patients with ectopia lentis and marfanoid habitus.The British journal of ophthalmology · 2002Article
- Marfan syndrome in the third Millennium.European journal of human genetics : EJHG · 2002Review
- Fibrillin: from microfibril assembly to biomechanical function.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2002Review
- Fibrillin-rich microfibrils: elastic biopolymers of the extracellular matrix.Journal of muscle research and cell motility · 2002Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Aggregation of fibrillin molecules via disulphide bonds is postulated to be an early step in microfibril assembly. By expressing fragments of fibrillin 1 and fibrillin 2 in a mammalian expression system, we found that the N-terminal region of each protein directs the formation of homodimers and that disulphide bonds stabilize this interaction. A large fragment of fibrillin 1 containing much of the region downstream from the N-terminus remained as a monomer when expressed in the same cell system, indicating that this region of the protein lacks dimerization domains. This finding also confirms that the overexpression of fibrillin fragments does not in itself lead to spurious dimer formation. Pulse-chase analysis demonstrated that dimer formation occurred intracellularly, suggesting that the process of fibrillin aggregation is initiated early after biosynthesis of the molecules. These findings also implicate the N-terminal region of fibrillin 1 and fibrillin 2 in directing the formation of a dimer intermediate that aggregates to form the functional microfibril.
Indexed as
Identifiers
10359653PMC1220300W2130103086What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.