ArticleMolecular biology of the cell1999
Direct visualization of a protein nuclear architecture.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 64 citations in OpenAlex.
- Physical Nature of Chromatin in the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- Ubiquitin C-terminal hydrolase isozyme L1 is associated with shelterin complex at interstitial telomeric sites.Epigenetics & chromatin · 2017Article
- Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI).Journal of visualized experiments : JoVE · 2015Article
- Cellular Levels of Signaling Factors Are Sensed by β-actin Alleles to Modulate Transcriptional Pulse Intensity.Cell reports · 2015Article
- Relationship between DNA replication and the nuclear matrix.Genes to cells : devoted to molecular & cellular mechanisms · 2013Review
- Ultrastructural study of transcription factories in mouse erythroblasts.Journal of cell science · 2011Article
- Elucidating chromatin and nuclear domain architecture with electron spectroscopic imaging.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Review
- Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks.The Journal of cell biology · 2006Article
- NMPdb: Database of Nuclear Matrix Proteins.Nucleic acids research · 2005Article
- Lamin A/C speckles mediate spatial organization of splicing factor compartments and RNA polymerase II transcription.The Journal of cell biology · 2002Article
- The transcription coactivator CBP is a dynamic component of the promyelocytic leukemia nuclear body.The Journal of cell biology · 2001Article
- Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments.The Journal of cell biology · 2000Article
- Mitotic transcription repression in vivo in the absence of nucleosomal chromatin condensation.The Journal of cell biology · 2000Article
- Spatial organization of RNA polymerase II transcription in the nucleus.Nucleic acids research · 2000Review
- Half a century of "the nuclear matrix".Molecular biology of the cell · 2000Review
- Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA.The Journal of cell biology · 2000Article
- A role for the GSG domain in localizing Sam68 to novel nuclear structures in cancer cell lines.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whether the cell nucleus is organized by an underlying architecture analagous to the cytoskeleton has been a highly contentious issue since the original isolation of a nuclease and salt-resistant nuclear matrix. Despite electron microscopy studies that show that a nuclear architecture can be visualized after fractionation, the necessity to elute chromatin to visualize this structure has hindered general acceptance of a karyoskeleton. Using an analytical electron microscopy method capable of quantitative elemental analysis, electron spectroscopic imaging, we show that the majority of the fine structure within interchromatin regions of the cell nucleus in fixed whole cells is not nucleoprotein. Rather, this fine structure is compositionally similar to known protein-based cellular structures of the cytoplasm. This study is the first demonstration of a protein network in unfractionated and uninfected cells and provides a method for the ultrastructural characterization of the interaction of this protein architecture with chromatin and ribonucleoprotein elements of the cell nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.