ArticleMolecular biology of the cell1999
High-voltage electron tomography of spindle pole bodies and early mitotic spindles in the yeast Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.
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Who cites it
149 citing papers in PubMed, 285 citations in OpenAlex.
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- Spindle architecture constrains karyotype evolution.Nature cell biology · 2024Article
- Spindle architecture constrains karyotype in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.Nature cell biology · 2023Article
- Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast.Journal of cell science · 2022Article
- Polymer models reveal how chromatin modification can modulate force at the kinetochore.Molecular biology of the cell · 2022Article
- The microtubule plus-end tracking protein Bik1 is required for chromosome congression.Molecular biology of the cell · 2022Article
- Moonlighting at the Poles: Non-Canonical Functions of Centrosomes.Frontiers in cell and developmental biology · 2022Review
- Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.Molecular biology of the cell · 2021Article
- Attenuated Chromosome Oscillation as a Cause of Chromosomal Instability in Cancer Cells.Cancers · 2021Review
- Aurora B Tension Sensing Mechanisms in the Kinetochore Ensure Accurate Chromosome Segregation.International journal of molecular sciences · 2021Review
- Mechanisms by Which Kinesin-5 Motors Perform Their Multiple Intracellular Functions.International journal of molecular sciences · 2021Review
- Microtubule pivoting enables mitotic spindle assembly in S. cerevisiae.The Journal of cell biology · 2021Article
- Polymer modelling unveils the roles of heterochromatin and nucleolar organizing regions in shaping 3D genome organization in Arabidopsis thaliana.Nucleic acids research · 2021Article
- Anatomy of the fungal microtubule organizing center, the spindle pole body.Current opinion in structural biology · 2021Review
- Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock.International journal of molecular sciences · 2021Article
- Ase1 domains dynamically slow anaphase spindle elongation and recruit Bim1 to the midzone.Molecular biology of the cell · 2020Article
89 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The spindle pole body (SPB) is the major microtubule-organizing center of budding yeast and is the functional equivalent of the centrosome in higher eukaryotic cells. We used fast-frozen, freeze-substituted cells in conjunction with high-voltage electron tomography to study the fine structure of the SPB and the events of early spindle formation. Individual structures were imaged at 5-10 nm resolution in three dimensions, significantly better than can be achieved by serial section electron microscopy. The SPB is organized in distinct but coupled layers, two of which show ordered two-dimensional packing. The SPB central plaque is anchored in the nuclear envelope with hook-like structures. The minus ends of nuclear microtubules (MTs) are capped and are tethered to the SPB inner plaque, whereas the majority of MT plus ends show a distinct flaring. Unbudded cells containing a single SPB retain 16 MTs, enough to attach to each of the expected 16 chromosomes. Their median length is approximately 150 nm. MTs growing from duplicated but not separated SPBs have a median length of approximately 130 nm and interdigitate over the bridge that connects the SPBs. As a bipolar spindle is formed, the median MT length increases to approximately 300 nm and then decreases to approximately 30 nm in late anaphase. Three-dimensional models confirm that there is no conventional metaphase and that anaphase A occurs. These studies complement and extend what is known about the three-dimensional structure of the yeast mitotic spindle and further our understanding of the organization of the SPB in intact cells.
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