ArticleMolecular biology of the cell1999
Microtubule-based endoplasmic reticulum motility in Xenopus laevis: activation of membrane-associated kinesin during development.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization.The Journal of cell biology · 2025Article
- Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation.Nature communications · 2025Article
- Making the connection: How membrane contact sites have changed our view of organelle biology.Cell · 2024Review
- The interconnection of endoplasmic reticulum and microtubule and its implication in Hereditary Spastic Paraplegia.Computational and structural biotechnology journal · 2023Review
- Dynein-dependent collection of membranes defines the architecture and position of microtubule asters in isolated, geometrically confined volumes of cell-free extracts.Molecular biology of the cell · 2022Article
- Tubulin Isotypes: Emerging Roles in Defining Cancer Stem Cell Niche.Frontiers in immunology · 2022Review
- Intertwined and Finely Balanced: Endoplasmic Reticulum Morphology, Dynamics, Function, and Diseases.Cells · 2021Review
- Network organisation and the dynamics of tubules in the endoplasmic reticulum.Scientific reports · 2021Article
- Article
- Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic Microtubules.Current biology : CB · 2020Article
- Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts.The Journal of cell biology · 2018Article
- The endoplasmic reticulum: structure, function and response to cellular signaling.Cellular and molecular life sciences : CMLS · 2016Review
- Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion.The Journal of cell biology · 2014Article
- Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts.The Journal of cell biology · 2013Article
- Dynamic microtubules and endomembrane cycling contribute to polarity establishment and early development of Ectocarpus mitospores.Protoplasma · 2013Article
- Mitotic inheritance of endoplasmic reticulum in the primitive red alga Cyanidioschyzon merolae.Protoplasma · 2012Article
- Calcineurin homologous protein: a multifunctional Ca2+-binding protein family.American journal of physiology. Renal physiology · 2012Review
- Unconventional functions of microtubule motors.Archives of biochemistry and biophysics · 2012Review
- Regulation of mouse oocyte microtubule and organelle dynamics by PADI6 and the cytoplasmic lattices.Developmental biology · 2011Article
- Plus- and minus-end directed microtubule motors bind simultaneously to herpes simplex virus capsids using different inner tegument structures.PLoS pathogens · 2010Article
Corrections and comments
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2 authors.
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Abstract
The endoplasmic reticulum (ER) in animal cells uses microtubule motor proteins to adopt and maintain its extended, reticular organization. Although the orientation of microtubules in many somatic cell types predicts that the ER should move toward microtubule plus ends, motor-dependent ER motility reconstituted in extracts of Xenopus laevis eggs is exclusively a minus end-directed, cytoplasmic dynein-driven process. We have used Xenopus egg, embryo, and somatic Xenopus tissue culture cell (XTC) extracts to study ER motility during embryonic development in Xenopus by video-enhanced differential interference contrast microscopy. Our results demonstrate that cytoplasmic dynein is the sole motor for microtubule-based ER motility throughout the early stages of development (up to at least the fifth embryonic interphase). When egg-derived ER membranes were incubated in somatic XTC cytosol, however, ER tubules moved in both directions along microtubules. Data from directionality assays suggest that plus end-directed ER tubule extensions contribute approximately 19% of the total microtubule-based ER motility under these conditions. In XTC extracts, the rate of ER tubule extensions toward microtubule plus ends is lower ( approximately 0.4 microm/s) than minus end-directed motility ( approximately 1.3 microm/s), and plus end-directed motility is eliminated by a function-blocking anti-conventional kinesin heavy chain antibody (SUK4). In addition, we provide evidence that the initiation of plus end-directed ER motility in somatic cytosol is likely to occur via activation of membrane-associated kinesin.
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Registered trials
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