ArticleMolecular biology of the cell1999
The interaction of activated integrin lymphocyte function-associated antigen 1 with ligand intercellular adhesion molecule 1 induces activation and redistribution of focal adhesion kinase and proline-rich tyrosine kinase 2 in T lymphocytes.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 81 citations in OpenAlex.
- Review
- miRNA-200c-3p targets talin-1 to regulate integrin-mediated cell adhesion.Scientific reports · 2021Article
- CD9 Controls Integrin α5β1-Mediated Cell Adhesion by Modulating Its Association With the Metalloproteinase ADAM17.Frontiers in immunology · 2018Article
- Micro-adhesion rings surrounding TCR microclusters are essential for T cell activation.The Journal of experimental medicine · 2016Article
- Functions of the FAK family kinases in T cells: beyond actin cytoskeletal rearrangement.Immunologic research · 2014Review
- Aldo-keto reductase family 1 member B8 is secreted via non-classical pathway.International journal of clinical and experimental pathology · 2014Article
- Chemokine CXCL12 uses CXCR4 and a signaling core formed by bifunctional Akt, extracellular signal-regulated kinase (ERK)1/2, and mammalian target of rapamycin complex 1 (mTORC1) proteins to control chemotaxis and survival simultaneously in mature dendritic cells.The Journal of biological chemistry · 2011Article
- Differential expression of FAK and Pyk2 in metastatic and non-metastatic EL4 lymphoma cell lines.Clinical & experimental metastasis · 2011Article
- T cell LFA-1 engagement induces HuR-dependent cytokine mRNA stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 signaling cascade.PloS one · 2010Article
- Cdc42-mediated MTOC polarization in dendritic cells controls targeted delivery of cytokines at the immune synapse.The Journal of experimental medicine · 2010Article
- Proline-rich tyrosine kinase-2 is critical for CD8 T-cell short-lived effector fate.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Sublethal concentrations of the platinum(II) complex [Pt(O,O'-acac)(gamma-acac)(DMS)] alter the motility and induce anoikis in MCF-7 cells.British journal of pharmacology · 2010Article
- Development of a platelet-activating factor antagonist for HIV-1 associated neurocognitive disorders.Journal of neuroimmunology · 2009Article
- SLP-76-ADAP adaptor module regulates LFA-1 mediated costimulation and T cell motility.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Dok-4 is a novel negative regulator of T cell activation.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Vav1 is essential for mechanotactic crawling and migration of neutrophils out of the inflamed microvasculature.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- LFA-1-mediated T cell costimulation through increased localization of TCR/class II complexes to the central supramolecular activation cluster and exclusion of CD45 from the immunological synapse.Journal of immunology (Baltimore, Md. : 1950) · 2007Article
- Article
- The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome.Nature cell biology · 2005Article
- Focal adhesion kinase-related protein tyrosine kinase Pyk2 in T-cell activation and function.Immunologic research · 2005Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integrin receptors play a central role in the biology of lymphocytes, mediating crucial functional aspects of these cells, including adhesion, activation, polarization, migration, and signaling. Here we report that induction of activation of the beta2-integrin lymphocyte function-associated antigen 1 (LFA-1) in T lymphocytes with divalent cations, phorbol esters, or stimulatory antibodies is followed by a dramatic polarization, resulting in a characteristic elongated morphology of the cells and the arrest of migrating lymphoblasts. This cellular polarization was prevented by treatment of cells with the specific tyrosine kinase inhibitor genistein. Furthermore, the interaction of the activated integrin LFA-1 with its ligand intercellular adhesion molecule 1 induced the activation of the cytoplasmic tyrosine kinases focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK-2). FAK activation reached a maximum after 45 min of stimulation; in contrast, PYK-2 activation peaked at 30 min, declining after 60 min. Upon polarization of lymphoblasts, FAK and PYK-2 redistributed from a diffuse localization in the cytoplasm to a region close to the microtubule-organizing center in these cells. FAK and PYK-2 activation was blocked when lymphoblasts were pretreated with actin and tubulin cytoskeleton-interfering agents, indicating its cytoskeletal dependence. Our results demonstrate that interaction of the beta2-integrin LFA-1 with its ligand intercellular adhesion molecule 1 induces remodeling of T lymphocyte morphology and activation and redistribution of the cytoplasmic tyrosine kinases FAK and PYK-2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.