ArticleMolecular biology of the cell1999
The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 157 citations in OpenAlex.
- SNARE chaperone Sly1 directly mediates close-range vesicle tethering.The Journal of cell biology · 2024Article
- Role of Rabenosyn-5 and Rab5b in host cell cytosol uptake reveals conservation of endosomal transport in malaria parasites.PLoS biology · 2024Article
- Rab5 GTPases are required for optimal TORC2 function.The Journal of cell biology · 2019Article
- The NaMolecular biology of the cell · 2018Article
- Multivalent Rab interactions determine tether-mediated membrane fusion.Molecular biology of the cell · 2017Article
- Spatiotemporal dynamics of membrane remodeling and fusion proteins during endocytic transport.Molecular biology of the cell · 2015Article
- Rab family of GTPases.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Ypt/Rab GTPases: principles learned from yeast.Critical reviews in biochemistry and molecular biology · 2015Review
- A Vps21 endocytic module regulates autophagy.Molecular biology of the cell · 2014Article
- Function and regulation of the endosomal fusion and fission machineries.Cold Spring Harbor perspectives in biology · 2014Review
- A congenital neutrophil defect syndrome associated with mutations in VPS45.The New England journal of medicine · 2013Article
- The CORVET complex promotes tethering and fusion of Rab5/Vps21-positive membranes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids.Journal of lipid research · 2013Article
- Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis.The Journal of biological chemistry · 2013Article
- A conserved regulatory mode in exocytic membrane fusion revealed by Mso1p membrane interactions.Molecular biology of the cell · 2013Article
- The N-terminal domains of Vps3 and Vps8 are critical for localization and function of the CORVET tethering complex on endosomes.PloS one · 2013Article
- Class E compartments form in response to ESCRT dysfunction in yeast due to hyperactivity of the Vps21 Rab GTPase.Journal of cell science · 2012Article
- Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3.Traffic (Copenhagen, Denmark) · 2012Article
- Discovery of novel vitamin D receptor interacting proteins that modulate 1,25-dihydroxyvitamin D3 signaling.The Journal of steroid biochemistry and molecular biology · 2012Article
- The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles.Molecular biology of the cell · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Activated GTP-bound Rab proteins are thought to interact with effectors to elicit vesicle targeting and fusion events. Vesicle-associated v-SNARE and target membrane t-SNARE proteins are also involved in vesicular transport. Little is known about the functional relationship between Rabs and SNARE protein complexes. We have constructed an activated allele of VPS21, a yeast Rab protein involved in vacuolar protein sorting, and demonstrated an allele-specific interaction between Vps21p and Vac1p. Vac1p was found to bind the Sec1p homologue Vps45p. Although no association between Vps21p and Vps45p was seen, a genetic interaction between VPS21 and VPS45 was observed. Vac1p contains a zinc-binding FYVE finger that may bind phosphatidylinositol 3-phosphate [PtdIns(3)P]. In other FYVE domain proteins, this motif and PtdIns(3)P are necessary for membrane association. Vac1 proteins with mutant FYVE fingers still associated with membranes but showed vacuolar protein sorting defects and reduced interactions with Vps45p and activated Vps21p. Vac1p membrane association was not dependent on PtdIns(3)P, Pep12p, Vps21p, Vps45p, or the PtdIns 3-kinase, Vps34p. Vac1p FYVE finger mutant missorting phenotypes were suppressed by a defective allele of VPS34. These data indicate that PtdIns(3)P may perform a regulatory role, possibly involved in mediating Vac1p protein-protein interactions. We propose that activated-Vps21p interacts with its effector, Vac1p, which interacts with Vps45p to regulate the Golgi to endosome SNARE complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.