ArticleMolecular biology of the cell1999
Membrane tubule-mediated reassembly and maintenance of the Golgi complex is disrupted by phospholipase A2 antagonists.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 70 citations in OpenAlex.
- PLA2G6-associated late-onset parkinsonism in a Sudanese family.Annals of clinical and translational neurology · 2023Article
- Golgi-Targeting Anticancer Natural Products.Cancers · 2023Review
- Endocytosis and Exocytosis inFrontiers in cellular and infection microbiology · 2020Article
- Hypoxia-mediated impaired erythrocyte Lands' Cycle is pathogenic for sickle cell disease.Scientific reports · 2016Article
- ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Methods for analyzing the role of phospholipase A₂ enzymes in endosome membrane tubule formation.Methods in cell biology · 2015Article
- Gβ1γ2 activates phospholipase AFrontiers in cell and developmental biology · 2014Article
- PAFAH Ib phospholipase A2 subunits have distinct roles in maintaining Golgi structure and function.Biochimica et biophysica acta · 2013Article
- Regulation of the Golgi complex by phospholipid remodeling enzymes.Biochimica et biophysica acta · 2012Review
- A PLA1-2 punch regulates the Golgi complex.Trends in cell biology · 2012Review
- Cohen syndrome-associated protein, COH1, is a novel, giant Golgi matrix protein required for Golgi integrity.The Journal of biological chemistry · 2011Article
- Article
- The phospholipase A₂ enzyme complex PAFAH Ib mediates endosomal membrane tubule formation and trafficking.Molecular biology of the cell · 2011Article
- A role for phospholipase A2 activity in membrane tubule formation and TGN trafficking.Traffic (Copenhagen, Denmark) · 2010Article
- Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretion.The Journal of cell biology · 2010Article
- The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex.The Journal of cell biology · 2010Article
- Phospholipase A(2) is required for PIN-FORMED protein trafficking to the plasma membrane in the Arabidopsis root.The Plant cell · 2010Article
- Golgi-modifying properties of macfarlandin E and the synthesis and evaluation of its 2,7-dioxabicyclo[3.2.1]octan-3-one core.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Assembly of an intact Golgi complex requires phospholipase A2 (PLA2) activity, membrane tubules, and dynein-mediated microtubule transport.Biochemical and biophysical research communications · 2009Article
- Golgi-associated cPLA2alpha regulates endothelial cell-cell junction integrity by controlling the trafficking of transmembrane junction proteins.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although membrane tubules can be found extending from, and associated with, the Golgi complex of eukaryotic cells, their physiological function has remained unclear. To gain insight into the biological significance of membrane tubules, we have developed methods for selectively preventing their formation. We show here that a broad range of phospholipase A2 (PLA2) antagonists not only arrest membrane tubule-mediated events that occur late in the assembly of the Golgi complex but also perturb its normal steady-state tubulovesicular architecture by inducing a reversible fragmentation into separate "mini-stacks." In addition, we show that these same compounds prevent the formation of membrane tubules from Golgi stacks in an in vitro reconstitution system. This in vitro assay was further used to demonstrate that the relevant PLA2 activity originates from the cytoplasm. Taken together, these results demonstrate that Golgi membrane tubules, sensitive to potent and selective PLA2 antagonists, mediate both late events in the reassembly of the Golgi complex and the dynamic maintenance of its steady-state architecture. In addition, they implicate a role for cytoplasmic PLA2 enzymes in mediating these membrane trafficking events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.